干扰素α(IFNa)活性蛋白

Active Interferon Alpha (IFNa)

IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D

  • 干扰素α(IFNa)活性蛋白 产品包装(模拟)
  • 干扰素α(IFNa)活性蛋白 产品包装(模拟)
  • 干扰素α(IFNa)活性蛋白 Gene sequencing
  • APA033Mu61.jpg SDS-PAGE图
  • 干扰素α(IFNa)活性蛋白 Figure. Western Blot; Sample: Recombinant IFNa, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

The Interferon-alpha (IFNa) proteins belong to type I interferons (IFNs) which a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNa proteins produced by leukocytes are also known as leukocyte interferon. They are mainly involved in innate immune response against viral infection. Besides, Interferon-alpha 13 (IFNA13) has been identified as an interactor of IFNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IFNa and recombinant mouse IFNA13. Briefly, IFNA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IFNA13-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IFNapAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IFNa and IFNA13 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
EPA033Mu61 干扰素α(IFNa)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA033Mu01 干扰素α(IFNa)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA033Mu61 干扰素α(IFNa)活性蛋白 Cell culture; Activity Assays.
PAA033Mu06 干扰素α(IFNa)多克隆抗体 WB; IHC; ICC; IP.
PAA033Mu01 干扰素α(IFNa)多克隆抗体 WB; IHC; ICC; IP.
LAA033Mu71 干扰素α(IFNa)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA033Mu72 干扰素α(IFNa)单克隆抗体(生物素标记) WB; IHC; ICC.
LAA033Mu76 干扰素α(IFNa)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA033Mu 干扰素α(IFNa)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA033Mu 干扰素α(IFNa)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA033Mu 干扰素α(IFNa)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA033Mu 干扰素α(IFNa)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA033Mu01 干扰素α(IFNa)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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