干扰素β(IFNb)活性蛋白

Active Interferon Beta (IFNb)

IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast

  • 干扰素β(IFNb)活性蛋白 产品包装(模拟)
  • 干扰素β(IFNb)活性蛋白 产品包装(模拟)
  • APA222Po02.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Interferon Beta (IFNb) is belongs to type I interferons (IFNs) family which a large subgroup of interferon proteins that help regulate the activity of the immune system.The IFNb proteins are produced in large quantities by fibroblasts. They have antiviral activity that is involved mainly in innate immune response. Two types of IFNb have been described, IFNb1 (IFNB1) and IFNb3 (IFNB3). IFNb1 is used as a treatment for multiple sclerosis as it reduces the relapse rate. To test the effect of IFNb on cell apoptosis, A549 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 5% serum standard DMEM  prior to the addition of various concentrations of recombinant pig IFNb. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Apoptosis of A549 cells after incubation with IFNb for 48h observed by inverted microscope was shown in Figure1. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant IFNb for 48h. The result was shown in Figure2. It was obvious that IFNb significantly decreased cell viability of A549 cells. The ED50 of recombinant pig IFNb is 4.9 ug/ml.

Figure 2. Cell apoptosis of A549 cells after stimulated with IFNb.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Sus scrofa; Porcine (Pig,猪) 应用(仅供研究使用,不用于临床诊断!)
EPA222Po61 干扰素β(IFNb)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA222Po02 干扰素β(IFNb)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA222Po01 干扰素β(IFNb)活性蛋白 Cell culture; Activity Assays.
APA222Po02 干扰素β(IFNb)活性蛋白 Cell culture; Activity Assays.
RPA222Po01 干扰素β(IFNb)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA222Po61 干扰素β(IFNb)活性蛋白 Cell culture; Activity Assays.
PAA222Po01 干扰素β(IFNb)多克隆抗体 WB; IHC; ICC; IP.
LAA222Po81 干扰素β(IFNb)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA222Po71 干扰素β(IFNb)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA222Po21 干扰素β(IFNb)单克隆抗体 WB; IHC; ICC; IP.
SEA222Po 干扰素β(IFNb)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA222Po 干扰素β(IFNb)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
20 Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathway [PubMed: 21355876]
Journal of General Virology Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3. [Sgm: 9182080]
Biochemical and Biophysical Research Communications Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damage [ScienceDirect: S0006291X09019822]
PLoS One Intact Type I Interferon Production and IRF7 Function in Sooty Mangabeys [PubMed: PMC3757038]
PLoS Neglected Tropical Diseases Innate Immune Response to Rift Valley Fever Virus in Goats [PubMed: PMC3335883]
BioMed Research International Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages [Pubmed: 24288685]
Vet Microbiol. An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages. [Pubmed: 23850441]
Food and Chemical Toxicology Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in mice [Pubmed: 23535186]
Veterinary immunology and immunopathology Experimental model of equine alveolar macrophage stimulation with TLR ligands [Pubmed: 23815824]
American journal of respiratory cell and molecular biology Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages [Pubmed: 24502337]
FEBS Lett. Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunity [Pubmed:24530528]
Veterinary Research Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain [Pubmed:25106750]
PLOS Neglected Tropical Diseases MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production [Pubmed:25122182]
Veterinary Research The innate immune response of equine bronchial epithelial cells is altered by training [Pubmed:25595212]
molecular and celluar biology SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity [PubMed: 25939384]
Arch Virol Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virus [PubMed: 25797196]
J Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic Cells [PubMed: 26258404]
Virus PKR activation enhances replication of classical swine fever virus in PK-15 cells [PubMed: 25899421]
PLoS One siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro [Pubmed:26886455]
Scientific Reports Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4 [Pubmed:27090571]
Front Microbiol Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo [Pubmed:27252695]
Canadian Journal of Physiology and Pharmacology Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25. [pubmed:28134560]
International Journal of Molecular Medicine Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function [pubmed:27176664]
International Journal of Clinical and Experimental Pathology Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells [files:ijcep0036479.pdf]
Poultry Science ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages [pubmed:27486255]
Journal of Virology Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. [pubmed:]
Biochemical and Biophysical Research Communications MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B [pubmed:28342861]
Virology The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection [pubmed:28988058]
EMBO Journal Zika virus elicits inflammation to evade antiviral response by cleaving cGAS via NS1‐caspase‐1 axis [Pubmed:30065070]
Toxicology and applied pharmacology Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult mice [Pubmed:30017639]
Archives of Virology MAVS induces a host cell defense to inhibit CSFV infection [Pubmed:29556776]
Journal of virology Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activation [Pubmed: 30518647]
Veterinary Immunology and Immunopathology Molecular cloning and functional characterization of porcine 2′, 5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory?… [Doi: 10.1016/j.vetimm.2019.01.003]
Science Signaling HIPK2 is necessary for type I interferon–mediated antiviral immunity [Doi: 10.1126/scisignal.aau4604]
INTERNATIONAL MEDICAL RESEARCH Evaluation of serum interferons in patients with age-related macular degeneration [Pubmed: 31007694]
Veterinary Immunology and Immunopathology Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens []
International journal of biological macromolecules Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction [Pubmed: 31284005]
Journal of virology Marek's Disease Virus RLORF4 Inhibits Type I Interferon Production by Antagonizing NF-κB Activation [Pubmed: 31243133]
The Journal of Latvian Academy of Sciences Serodiagnosis of Human Bocavirus 1 Infection among Hospitalised Children with Lower Respiratory Tract Infection in Latvia []
Cancer Medicine The immunostimulatory effects and pro‐apoptotic activity of rhCNB against Lewis lung cancer is mediated by Toll‐like receptor 4 [Pubmed: 31218844]
FASEB Journal Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1 []
PLOS Pathogens Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion [Pubmed: 31539404]
Autophagy Induction of autophagy and suppression of type I IFN secretion by CSFV [Pubmed: 32160078]
MOLECULAR IMMUNOLOGY BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activation [Pubmed: 33053462]
Autophagy LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection [Pubmed: 32924761]
Arch Virol . Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway [Pubmed: 32532134]
Virulence Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon … []
bioRxiv Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance []
Res Vet Sci Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells [34416463]
Eur J Immunol Anti‐IFN‐α/‐ω neutralizing antibodies from COVID‐19 patients correlate with downregulation of IFN response and laboratory biomarkers of disease severity [Pubmed:35419822]
ResearchSquare Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I []
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