白介素1受体拮抗剂(IL1RA)活性蛋白

Active Interleukin 1 Receptor Antagonist (IL1RA)

IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra

  • 白介素1受体拮抗剂(IL1RA)活性蛋白 产品包装(模拟)
  • 白介素1受体拮抗剂(IL1RA)活性蛋白 产品包装(模拟)
  • 白介素1受体拮抗剂(IL1RA)活性蛋白 Gene sequencing
  • APA223Mu01.jpg SDS-PAGE图
  • 白介素1受体拮抗剂(IL1RA)活性蛋白 Figure. Western Blot; Sample: Recombinant IL1RA, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

IL1RA (interleukin-1 receptor antagonist) is an agent that binds to the cell surface interleukin-1 receptor (IL-1R), which would prevent IL-1 from intracellular signal transduction. Besides, mouse IL1R1 and rat IL1R1 share similarities in amino acid sequence with the identity of 85.6%. Thus, a binding ELISA assay was constructed to detect the association of recombinant mouse IL1RA with recombinant rat IL1R1. Briefly, IL1RA were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL1RA with IL1R1 was shown in Figure 1 and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
APA223Mu01 白介素1受体拮抗剂(IL1RA)活性蛋白 Cell culture; Activity Assays.
RPA223Mu02 白介素1受体拮抗剂(IL1RA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA223Mu01 白介素1受体拮抗剂(IL1RA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA223Mu02 白介素1受体拮抗剂(IL1RA)多克隆抗体 WB; IHC; ICC; IP.
PAA223Mu01 白介素1受体拮抗剂(IL1RA)多克隆抗体 WB; IHC; ICC; IP.
LAA223Mu71 白介素1受体拮抗剂(IL1RA)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA223Mu81 白介素1受体拮抗剂(IL1RA)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEA223Mu 白介素1受体拮抗剂(IL1RA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA223Mu01 白介素1受体拮抗剂(IL1RA)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
The Veterinary Journal Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease [ScienceDirect: S1090023312000962]
Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horses [Researchgate:Source]
Journal of Equine Veterinary Science Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1 [Science: Article]
Journal of Equine Veterinary Science A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol Concentrations [Science: Article]
Scientific publications in peer reviewed journals Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horse [receive:101658]
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