白介素17C(IL17C)活性蛋白

Active Interleukin 17C (IL17C)

Cytokine CX2

  • 白介素17C(IL17C)活性蛋白 产品包装(模拟)
  • 白介素17C(IL17C)活性蛋白 产品包装(模拟)
  • APD347Hu01.jpg Figure. SDS-PAGE
  • 白介素17C(IL17C)活性蛋白 Figure. Western Blot; Sample: Recombinant IL17C, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to play a crucial role in innate immunity of the epithelium. IL17C binds with affinity to IL17RA. Thus, a binding ELISA assay was constructed to detect the association of rhIL17C with IL17RA. Briefly, rhIL17C were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.
Figure. The binding activity of IL17C with IL17RA.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

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编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPD347Hu01 白介素17C(IL17C)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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