白介素17C(IL17C)活性蛋白

Active Interleukin 17C (IL17C)

Cytokine CX2

  • 白介素17C(IL17C)活性蛋白 产品包装(模拟)
  • 白介素17C(IL17C)活性蛋白 产品包装(模拟)
  • APD347Hu01.jpg Figure. SDS-PAGE
  • 白介素17C(IL17C)活性蛋白 Figure. Western Blot; Sample: Recombinant IL17C, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to play a crucial role in innate immunity of the epithelium. IL17C binds with affinity to IL17RA. Thus, a binding ELISA assay was constructed to detect the association of rhIL17C with IL17RA. Briefly, rhIL17C were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.
Figure. The binding activity of IL17C with IL17RA.

用法

PBS或其它。

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPD347Hu01 白介素17C(IL17C)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APD347Hu01 白介素17C(IL17C)活性蛋白 Cell culture; Activity Assays.
PAD347Hu01 白介素17C(IL17C)多克隆抗体 WB; IHC; ICC; IP.
MAD347Hu21 白介素17C(IL17C)单克隆抗体 WB; IHC; ICC; IP.
SED347Hu 白介素17C(IL17C)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCD347Hu 白介素17C(IL17C)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMD347Hu 白介素17C(IL17C)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Cell Mol Gastroenterol Hepatol Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis [Pubmed: 30716420]
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