白介素2受体α(IL2Ra)活性蛋白
Active Interleukin 2 Receptor Alpha (IL2Ra)
CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen
- 编号APB837Mu02
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点7.2
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1152 ¥ 2880 ¥ 5760 ¥ 17280 ¥ 43200
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产品包装(模拟)
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产品包装(模拟)
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SDS-PAGE图
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Figure. Western Blot; Sample: Recombinant IL2Ra, Mouse.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
IL2RA (Interleukin-2 receptor alpha chain ), also known as Tac antigen and as CD25, was initially identified as a 55kDa membrane glycoprotein which is capable of binding IL 2. Besides, mouse IL2 shares 75.1% AA sequence identity with rat IL2, suggesting the exist of cross-species activity. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant rat IL2RA with recombinant mouse IL2. Briefly, IL2RA were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL2RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL2RA with IL2 was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APB837Mu02 | 白介素2受体α(IL2Ra)活性蛋白 | Cell culture; Activity Assays. |
| RPB837Mu01 | 白介素2受体α(IL2Ra)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB837Mu02 | 白介素2受体α(IL2Ra)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB837Mu01 | 白介素2受体α(IL2Ra)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB837Mu02 | 白介素2受体α(IL2Ra)多克隆抗体 | WB; IHC; ICC; IP. |
| FAB837Mu01 | 抗白介素2受体α(IL2Ra)多克隆抗体 | Flow cytometry. |
| FAB837Mu41 | 抗白介素2受体α(IL2Ra)多克隆抗体(藻红素标记) | Flow cytometry. |
| FAB837Mu51 | 抗白介素2受体α(IL2Ra)多克隆抗体(别藻蓝蛋白标记) | Flow cytometry. |
| FAB837Mu81 | 抗白介素2受体α(IL2Ra)多克隆抗体(异硫氰酸荧光素标记) | Flow cytometry. |
| SEB837Mu | 白介素2受体α(IL2Ra)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB837Mu | 白介素2受体α(IL2Ra)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Microbiology, Immunology and Infection | Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients [pubmed:28655573] |
