Active Interleukin 20 (IL20)
IL10D; IL10-D; ZCYTO10; Cytokine Zcyto10
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 90%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1320 ¥ 3300 ¥ 6600 ¥ 19800 ¥ 49500
- Figure. SDS-PAGE; Sample: Active recombinant IL20, Human.
- Figure. Western Blot; Sample: Recombinant IL20, Human.
- 通过ISO 9001、ISO 13485质量体系认证
IL20 (Interleukin-20) is a cytokine structurally related to interleukin 10, which is produced by activated keratinocytes and monocytes. It is accepted that IL20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Thus, proliferation assay of IL20 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL20. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of ECV-304 cells after incubation with IIL20 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL20 for 48h. The result was shown in Figure 2. It was obvious that human IL20 significantly decreased cell viability of ECV-304 cells.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|RPC058Hu01||白介素20(IL20)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC058Hu01||白介素20(IL20)活性蛋白||Cell culture; Activity Assays.|
|PAC058Hu01||白介素20(IL20)多克隆抗体||WB; IHC; ICC; IP.|
|MAC058Hu22||白介素20(IL20)单克隆抗体||WB; IHC; ICC; IP.|
|SEC058Hu||白介素20(IL20)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCC058Hu||白介素20(IL20)检测试剂盒(化学发光免疫分析法)||Chemiluminescent immunoassay for Antigen Detection.|
|Molecular Oncology||The tumor promoting activity of the EP4 receptor for prostaglandin E2 in murine skin [Pubmed:25034079]|