白介素20(IL20)活性蛋白

Active Interleukin 20 (IL20)

IL10D; IL10-D; ZCYTO10; Cytokine Zcyto10

仅供体外研究使用,不用于临床诊断!警防假冒,可索取进口关税税单及报关单!
  • 白介素20(IL20)活性蛋白 产品包装(模拟)
  • 白介素20(IL20)活性蛋白 产品包装(模拟)
  • APC058Hu01.jpg Figure. SDS-PAGE; Sample: Active recombinant IL20, Human.
  • 白介素20(IL20)活性蛋白 Figure. Western Blot; Sample: Recombinant IL20, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

IL20 (Interleukin-20) is a cytokine structurally related to interleukin 10, which is produced by activated keratinocytes and monocytes. It is accepted that IL20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Thus, proliferation assay of IL20 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL20. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of ECV-304 cells after incubation with IIL20 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL20 for 48h. The result was shown in Figure 2. It was obvious that human IL20 significantly decreased cell viability of ECV-304 cells.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用
RPC058Hu01 白介素20(IL20)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC058Hu01 白介素20(IL20)活性蛋白 Cell culture; Activity Assays.
PAC058Hu01 白介素20(IL20)多克隆抗体 WB; IHC; ICC; IP.
MAC058Hu22 白介素20(IL20)单克隆抗体 WB; IHC; ICC; IP.
SEC058Hu 白介素20(IL20)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC058Hu 白介素20(IL20)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

参考文献

杂志 参考文献
Molecular Oncology The tumor promoting activity of the EP4 receptor for prostaglandin E2 in murine skin [Pubmed:25034079]
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