白介素24(IL24)活性蛋白

Active Interleukin 24 (IL24)

C49A; FISP; IL10B; MDA7; Mob-5; ST16; Mda-7; Melanoma Differentiation Association Protein 7; Suppression Of Tumorigenicity 16

  • 白介素24(IL24)活性蛋白 产品包装(模拟)
  • 白介素24(IL24)活性蛋白 产品包装(模拟)
  • APC064Hu01.jpg Figure. SDS-PAGE; Sample: Active recombinant IL24, Human.
  • 白介素24(IL24)活性蛋白 Figure. Western Blot ; Sample: Recombinant IL24, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can induce apoptosis selectively in various cancer cells, including ECV304. Thus, inhibition of cell proliferation assay of IL24 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL24. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Inhibition of ECV-304 cells proliferation after incubation with IIL24 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of IL24 for 48h. The mean OD value of ECV-304 assessed by CCK-8 was shown in Figure 2. It was obvious that IL24 significantly decreased cell viability of ECV-304 cells.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPC064Hu01 白介素24(IL24)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC064Hu01 白介素24(IL24)活性蛋白 Cell culture; Activity Assays.
PAC064Hu01 白介素24(IL24)多克隆抗体 WB; IHC; ICC; IP.
LAC064Hu71 白介素24(IL24)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC064Hu22 白介素24(IL24)单克隆抗体 WB; IHC; ICC; IP.
SEC064Hu 白介素24(IL24)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC064Hu 白介素24(IL24)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

参考文献

杂志 参考文献
Experimental & Clinical Cancer Research Oncolytic adenovirus armed with IL-24 Inhibits the growth of breast cancer in vitro and in vivo [PubMed: PMC3511263]
Bioresources and Bioprocessing Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies [Springer:Source]
Clin Exp Pharmacol Physiol. Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. [pubmed:28251688]
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