白介素5(IL5)活性蛋白

Active Interleukin 5 (IL5)

E-CSF; ESGF; EDF; TRF; Colony-Stimulating Factor,Eosinophil; T-Cell replacing Factor; B Cell Differentiation Factor I; Eosinophil Differentiation Factor

  • 白介素5(IL5)活性蛋白 产品包装(模拟)
  • 白介素5(IL5)活性蛋白 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Interleukin-5 (IL-5), a secreted glycoprotein, is a member of the hematopoietic family. Interleukin 5 has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 1x104cells/well and incubated for 48h in the presence or absence of various concentrations of IL-5 at 37oC. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.
Cell proliferation of TF-1 cells after incubation with IL-5 for 48h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of IL-5 was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 0.18 to 7.675ng/mL.

用法

Reconstitute in ddH2O to a concentration of 0.1-0.2 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
EPA078Hu61 白介素5(IL5)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA078Hu01 白介素5(IL5)活性蛋白 Cell culture; Activity Assays.
EPA078Hu62 白介素5(IL5)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA078Hu61 白介素5(IL5)活性蛋白 Cell culture; Activity Assays; In vivo assays.
EPA078Hu41 白介素5(IL5)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA078Hu01 白介素5(IL5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA078Hu01 白介素5(IL5)多克隆抗体 WB; IHC; ICC; IP.
FAA078Hu01 抗白介素5(IL5)多克隆抗体 Flow cytometry.
RAA078Hu21 白介素5(IL5)重组抗体 WB; IF; ICC; IHC; IP; FCM.
MAA078Hu21 白介素5(IL5)单克隆抗体 WB
MAA078Hu22 白介素5(IL5)单克隆抗体 WB
SEA078Hu 白介素5(IL5)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA078Hu 白介素5(IL5)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
IGA078Hu21 白介素5(IL5)人基因嵌入小鼠 动物实验，阳性对照。

参考文献

杂志 参考文献
Critical Care Medicine Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage [PubMed: PMC3383056]
veterinary research forum Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells [Article_14936_6.Html: ]
Molecular Immunology Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-ŚĘB in murine model of asthma [Pubmed:27289030]
Advances in Experimental Medicine and Biology Effects of Selective Inhibition of PDE4 by YM976 on Airway Reactivity and Cough in Ovalbumin-Sensitized Cavia (Guinea pig )inea Pigs [Pubmed:27130219]
Sci Rep. Interleukin-31 expression and relation to disease severity in human asthma [pmc:PMC4783779]
Molecular Immunology Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma [pubmed:27289030]
Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847]
Asian Pacific Society of Respirology Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397]
Acta Pharmacologica Sinica Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi') [Pubmed: 19363516]
International Journal of Peptide Research and Therapeutics ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma []
Journal of Pineal Research Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267]
Analytica Chimica Acta An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis []
American Journal of Translational Research Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193]
bioscience reports Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767]
Biomedicine & Pharmacotherapy Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice [Pubmed: 32521453]
Journal of Traditional Chinese Medical Sciences Mahuang Xixin Fuzi Decoction protects against ovalbumin-induced allergic rhinitis by inhibiting type 2 innate lymphoid cells in mice []
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota []
Annals of Tropical Medicine & Public Health Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq []
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies [33999928]
Mol Immunol MiR-142-5p directly targets cyclin-dependent kinase 5-mediated upregulation of the inflammatory process in acquired middle ear cholesteatoma [34875451]
Mljekarstvo Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows []
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