白介素8(IL8)活性蛋白

Active Interleukin 8 (IL8)

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

  • 白介素8(IL8)活性蛋白 产品包装(模拟)
  • 白介素8(IL8)活性蛋白 产品包装(模拟)
  • 白介素8(IL8)活性蛋白 Figure. Gene Sequencing (Extract)
  • APA080Hu01.png Figure. SDS-PAGE
  • 白介素8(IL8)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of IL8 with SDC1.
Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cellsendothelial cells. IL-8, also known as neutrophil chemotactic factor, has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL8 also induces phagocytosis once they have arrived. IL8 is also known to be a potent promoter of angiogenesis. Besides, Syndecan 1 (SDC1) has been identified as an interactor of IL8, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL8 and recombinant human SDC1. Briefly, IL8 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SDC1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL8 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL8 and SDC1 was shown in Figure 1, and this effect was in a dose dependent manner.

Figure. The chemotactic effect of IL8 on Jurkat cells
IL8 is a kind of neutrophil chemotactic factor. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of IL8 on the human T-lymphocyte leukemia cell line Jurkat. Briefly, Jurkat cells were seeded into the upper chambers (100μL cell suspension, 106cells/mL in RPMI 1640 with FBS free) and recombinant human IL8 (10ng/mL, 100ng/mL and 1000ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows IL8 is able to induce migration of Jurkat cells. The migrated Jurkat cells in low chamber at low magnification (×100) were shown in Figure 2. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 3. The optimum chemotaxis of IL8 occurs at 10~100ng/mL.
(A) Jurkat cells were seeded into the upper chambers and serum free RPMI 1640 with 10ng/mL IL8 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h;
(B) Jurkat cells were seeded into the upper chambers and serum free RPMI 1640 without IL8 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h.

Figure. The chemotactic effect of IL8 on Jurkat cells.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA080Hu01 白介素8(IL8)活性蛋白 Cell culture; Activity Assays.
RPA080Hu01 白介素8(IL8)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA080Hu01 白介素8(IL8)多克隆抗体 WB; IHC; ICC; IP.
LAA080Hu71 白介素8(IL8)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA080Hu81 白介素8(IL8)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA080Hu24 白介素8(IL8)单克隆抗体 WB; IHC; ICC; IP.
MAA080Hu22 白介素8(IL8)单克隆抗体 WB; IHC; ICC; IP.
LAA080Hu82 白介素8(IL8)单克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC.
LAA080Hu72 白介素8(IL8)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA080Hu 白介素8(IL8)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA080Hu 白介素8(IL8)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA080Hu 白介素8(IL8)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA080Hu 白介素8(IL8)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
PSA080Hu01 白介素8(IL8)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA080Hu01 白介素8(IL8)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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