可罗索(KL)活性蛋白

Active Klotho (KL)

KLa; Klotho Alpha

  • 可罗索(KL)活性蛋白 产品包装(模拟)
  • 可罗索(KL)活性蛋白 产品包装(模拟)
  • 可罗索(KL)活性蛋白 Figure. Gene Sequencing (Extract)
  • APH757Ra01.png Figure. SDS-PAGE
  • 可罗索(KL)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Klotho (KL) is a transmembrane protein that, in addition to other effects, provides some control over the sensitivity of the organism to insulin and appears to be involved in aging. The Klotho protein is a novel β-glucuronidase capable of hydrolyzing steroid β-glucuronides. Genetic variants in KLOTHO have been associated with human aging, and Klotho protein has been shown to be a circulating factor detectable in serum that declines with age. The binding of certain fibroblast growth factors (FGF's) viz., FGF19, FGF20, and FGF23, to their fibroblast growth factor receptors, is promoted via their interactions as co-receptors with β-Klotho. Besides, Fibroblast Growth Factor 23 (FGF23) has been identified as an interactor of KL, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat KL and recombinant rat FGF23. Briefly, KL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to FGF23-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-KL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of KL and FGF23 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of KL with FGF23.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APH757Ra01 可罗索(KL)活性蛋白 Cell culture; Activity Assays.
RPH757Ra02 可罗索(KL)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPH757Ra01 可罗索(KL)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAH757Ra02 可罗索(KL)多克隆抗体 WB; IHC; ICC; IP.
PAH757Ra01 可罗索(KL)多克隆抗体 WB; IHC; ICC; IP.
MAH757Ra21 可罗索(KL)单克隆抗体 WB; IHC; ICC; IP.
SEH757Ra 可罗索(KL)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMH757Ra 可罗索(KL)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Life Sciences High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculature [Pubmed: 24012609]
EXPERIMENTAL & CLINICAL CARDIOLOGY Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice [Com:Source]
Int J Clin Exp Pathol.? α-Klotho is an acute phase protein and altered by restraint stress in mice [Pubmed:Pmc4203207]
Annales de Biologie Clinique Les biomarqueurs des calcifications vasculaires [PubMed: 26069069]
Mol Med Rep Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with Klotho [PubMed: 25873020]
Calcif Tissue Int Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3 [PubMed: 25899975]
中华内分泌代谢杂志 抗衰老蛋白Klotho 阻断高糖培养的大鼠肾小球系膜细胞TLR4/NF-κB p65/NGAL 通路的激活及其作用机制 [Cn: Article]
Mol Cell Endocrinol The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism [PubMed: 26419931]
Arch Med Sci Soluble Klotho protein as a novel serum biomarker in patients with acromegaly [pmc:PMC4754356]
Am J Nephrol Klotho Deficiency Aggravates Tacrolimus-Induced Renal Injury via the Phosphatidylinositol 3-Kinase-Akt-Forkhead Box Protein O Pathway [Pubmed:27174564]
Sci Rep. Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice. [pubmed:28387374]
Nephrology Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management? [pubmed:28429560]
Journal of Integrative Nephrology & Andrology Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patients [issn:2394-2916]
International Journal of Clinical and Experimental Pathology Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in mice [ISSN:1936-2625]
Journal of veterinary medical science Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat model [pubmed:28943597]
Cellular Physiology and Biochemistry Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury [Pubmed:29550818]
American Journal of Translational Research Advanced maternal age impairs spatial learning capacity in young adult mouse offspring [Pubmed:29636887]
Journal of Diabetes and its Complications Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complications [Pubmed:30042059]
Medical Science Monitor Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal … [Pubmed:30004089]
Nephrology Dialysis Transplantation High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease [Pubmed: 30189026]
BMC Nephrology The role of klotho in chronic kidney disease [Pubmed: 30348110]
Nature Communications Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regeneration [Pubmed: 30451844]
Kidney International Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome [Pubmed: 30770217]
Expert Opinion on Therapeutic Targets The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic rats [Pubmed: 30801205]
Mol Immunol CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection [Pubmed: 33223223]
Journal of Clinical Hypertension Effects of salt intervention on serum levels of Klotho influenced by salt sensitivity [Pubmed: 33164306]
CURRENT EYE RESEARCH Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic rats [Pubmed: 32744464]
international immunopharmacology Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats [Pubmed: 32438076]
Purinergic Signalling P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy. [Pubmed: 32377919]
Oxidative Medicine and Cellular Longevity Ameliorating Effect of Klotho Protein on Rat Heart during I/R Injury [Pubmed: 33123314]
NEUROSCIENCE Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With Schizophrenia [Pubmed: 32612508]
Pharmacological Reports ¦ÁKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy [33675964]
J Mol Neurosci Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma Cells [Pubmed:35112226]
Reproductive Biology and Endocrinology Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance []
Journal of Ovarian Research Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome []
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