卵磷脂胆固醇脂酰转移酶(LCAT)活性蛋白

Active Lecithin Cholesterol Acyltransferase (LCAT)

phosphatidylcholine-sterol O-acyltransferase; Phospholipid-cholesterol acyltransferase

  • 卵磷脂胆固醇脂酰转移酶(LCAT)活性蛋白 产品包装(模拟)
  • 卵磷脂胆固醇脂酰转移酶(LCAT)活性蛋白 产品包装(模拟)
  • 卵磷脂胆固醇脂酰转移酶(LCAT)活性蛋白 Figure . Gene Sequencing (extract)
  • APJ516Mu01.jpg Figure. SDS-PAGE; Sample: Active recombinant LCAT, Mouse.
  • 卵磷脂胆固醇脂酰转移酶(LCAT)活性蛋白 Figure. Western Blot; Sample: Recombinant LCAT, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

LCAT (Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that APOA1 (Apolipoprotein A-I) acts as a cofactor for the LCAT. Thus, a binding ELISA assay was conducted to detect the association of LCAT with APOA1. Briefly, recombinant rat LCAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APOA1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with APOA1 was shown in Figure 1 and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPJ516Mu02 卵磷脂胆固醇脂酰转移酶(LCAT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPJ516Mu01 卵磷脂胆固醇脂酰转移酶(LCAT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APJ516Mu01 卵磷脂胆固醇脂酰转移酶(LCAT)活性蛋白 Cell culture; Activity Assays.
PAJ516Mu01 卵磷脂胆固醇脂酰转移酶(LCAT)多克隆抗体 WB; IHC; ICC; IP.
PAJ516Mu02 卵磷脂胆固醇脂酰转移酶(LCAT)多克隆抗体 WB; IHC; ICC; IP.
LAJ516Mu71 卵磷脂胆固醇脂酰转移酶(LCAT)多克隆抗体(生物素标记) WB; IHC; ICC.
SEJ516Mu 卵磷脂胆固醇脂酰转移酶(LCAT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ516Mu 卵磷脂胆固醇脂酰转移酶(LCAT)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Int J Rheum Dis Plasma phospholipase, γ‐CEHC and antioxidant capacity in fibromyalgia [PubMed: 26585319]
Inflammation. Alteration of Lysophosphatidylcholine-Related Metabolic Parameters in the Plasma of Mice withExperimental Sepsis. [pubmed:28028754]
British journal of cancer A combined biomarker panel shows improved sensitivity for the early detection of ovarian cancer allowing the identification of the most aggressive type II tumours. [pubmed:28664912]
Journal of Agricultural and Food Chemistry Lipid-lowering effects of medium-chain triglyceride-enriched coconut oil in combination with licorice extracts in experimental hyperlipidemic mice [Pubmed: 30244576]
Experimental and Therapeutic Medicine Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D‑LC‑MS/MS [Doi: 10.3892/etm.2018.6818]
British Journal of Cancer Diagnosis of epithelial ovarian cancer using a combined protein biomarker panel. [Pubmed: 31388184]
BIOMEDICINE & PHARMACOTHERAPY The synergistic mechanism of total saponins and flavonoids in Notoginseng− Safflower pair against myocardial ischemia uncovered by an integrated … [Pubmed: 32739736]
Biomedicine & Pharmacotherapy Pharmacokinetics/pharmacometabolomics-pharmacodynamics reveals the synergistic mechanism of a multicomponent herbal formula, Baoyuan decoction?¡­ []
Cell Metab Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression [Pubmed:35235775]
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