白血病抑制因子(LIF)活性蛋白

Active Leukemia Inhibitory Factor (LIF)

CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin

  • 白血病抑制因子(LIF)活性蛋白 产品包装(模拟)
  • 白血病抑制因子(LIF)活性蛋白 产品包装(模拟)
  • 白血病抑制因子(LIF)活性蛋白 Gene sequencing
  • APA085Hu01.jpg Figure. SDS-PAGE
  • 白血病抑制因子(LIF)活性蛋白 Figure. Western Blot; Sample: Recombinant LIF, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. Cell proliferation of TF-1 cells after stimulated with LIF.
Leukemia Inhibitory Factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. To test the effect of LIF on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human LIF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of TF-1 cells after incubation with LIF for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant LIF for 72h. The result was shown in Figure 2. It was obvious that LIF significantly increased cell viability of TF-1 cells.
(A) TF-1 cells cultured in 1640, stimulated with 0.5ng/mL LIF for 72h;
(B) Unstimulated TF-1 cells cultured in 1640 for 72h.

Figure. Cell proliferation of TF-1 cells after stimulated with LIF.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA085Hu01 白血病抑制因子(LIF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA085Hu01 白血病抑制因子(LIF)活性蛋白 Cell culture; Activity Assays.
PAA085Hu01 白血病抑制因子(LIF)多克隆抗体 WB; IHC; ICC; IP.
MAA085Hu22 白血病抑制因子(LIF)单克隆抗体 WB; IHC; ICC; IP.
SEA085Hu 白血病抑制因子(LIF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
British Journal of Anaesthesia Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids [pubmed:28077540]
Biological Rhythm Research A study of altered cytokine rhythms associated with successful implantation in cows [10.1080/09291016.2017.1361159]
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