白血病抑制因子(LIF)活性蛋白

Active Leukemia Inhibitory Factor (LIF)

CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin

  • 白血病抑制因子(LIF)活性蛋白 产品包装(模拟)
  • 白血病抑制因子(LIF)活性蛋白 产品包装(模拟)
  • 白血病抑制因子(LIF)活性蛋白 Gene sequencing
  • APA085Hu01.jpg SDS-PAGE图
  • 白血病抑制因子(LIF)活性蛋白 Figure. Western Blot; Sample: Recombinant LIF, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Leukemia inhibitory factor (LIF) is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 1x104 cells/well and incubated for 72h in the presence or absence of various concentrations of LIF at 37°C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 °C.
Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of LIF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 0.05 to 1.67ng/mL

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA085Hu01 白血病抑制因子(LIF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA085Hu01 白血病抑制因子(LIF)活性蛋白 Cell culture; Activity Assays.
PAA085Hu01 白血病抑制因子(LIF)多克隆抗体 WB; IHC; ICC; IP.
MAA085Hu22 白血病抑制因子(LIF)单克隆抗体 WB; IHC; ICC; IP.
SEA085Hu 白血病抑制因子(LIF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
British Journal of Anaesthesia Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids [pubmed:28077540]
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