Active Leukemia Inhibitory Factor (LIF)
CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 品牌商CLOUD-CLONE CORP.(CCC, USA)
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 1g (保证有货 万一缺货 免费重做)
- 价格 ¥ 1843 ¥ 4608 ¥ 9216 ¥ 27648 ¥ 691200
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant LIF, Human.
- 通过ISO 9001、ISO 13485质量体系认证
Leukemia inhibitory factor (LIF) is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 1x104 cells/well and incubated for 72h in the presence or absence of various concentrations of LIF at 37°C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 °C.
Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1.
The dose-effect curve of LIF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 0.05 to 1.67ng/mL
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用|
|RPA085Hu01||白血病抑制因子(LIF)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA085Hu01||白血病抑制因子(LIF)活性蛋白||Cell culture; Activity Assays.|
|PAA085Hu01||白血病抑制因子(LIF)多克隆抗体||WB; IHC; ICC; IP.|
|MAA085Hu22||白血病抑制因子(LIF)单克隆抗体||WB; IHC; ICC; IP.|
|SEA085Hu||白血病抑制因子(LIF)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|British Journal of Anaesthesia||Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids [pubmed:28077540]|