赖氨酰氧化酶(LOX)活性蛋白

Active Lysyl Oxidase (LOX)

Protein-Lysine 6-Oxidase

仅供体外研究使用,不用于临床诊断!警防假冒,可索取进口关税税单及报关单!
  • 赖氨酰氧化酶(LOX)活性蛋白 产品包装(模拟)
  • 赖氨酰氧化酶(LOX)活性蛋白 产品包装(模拟)
  • APC580Hu01.jpg SDS-PAGE图
  • 赖氨酰氧化酶(LOX)活性蛋白 Figure. Western Blot; Sample: Recombinant LOX, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Lysyl oxidase (LOX) also known as protein-lysine 6-oxidase is an extracellular copper-dependent enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors. Its catalytic activity depends upon both its copper cofactor and a unique carbonyl cofactor and has been shown to extend to a variety of basic globular proteins, including histone H1. LOX plays a major role in connective tissue development and may also be important in neurological function. Lysyl oxidase has also proven crucial to the development of the respiratory system and the skin, the commitment step of adipocyte, and the formation of pluripotent stem cells during development. Its absence may lead to defects in the transforming growth factor beta superfamily of proteins, which control cell growth and differentiation. Besides, Elastin (ELN) has been identified as an interactor of LOX, thus a binding ELISA assay was conducted to detect the interaction of recombinant human LOX and recombinant human ELN. Briefly, LOX were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ELN-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-LOX pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of LOX and ELN was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用
APC580Hu01 赖氨酰氧化酶(LOX)活性蛋白 Cell culture; Activity Assays.
RPC580Hu01 赖氨酰氧化酶(LOX)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC580Hu01 赖氨酰氧化酶(LOX)多克隆抗体 WB; IHC; ICC; IP.
LAC580Hu81 赖氨酰氧化酶(LOX)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAC580Hu71 赖氨酰氧化酶(LOX)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC580Hu22 赖氨酰氧化酶(LOX)单克隆抗体 WB; IHC; ICC; IP.
SEC580Hu 赖氨酰氧化酶(LOX)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEC580Hu 赖氨酰氧化酶(LOX)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
KSC580Hu01 赖氨酰氧化酶(LOX)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
American Journal of Hematology The expression of lysyl-oxidase gene family members in myeloproliferative neoplasms. [PubMed: 23494965]
Current Eye Research High Glucose Induced Differential Expression of Lysyl Oxidase and Its Isoform in ARPE-19 Cells [Pubmed: 22966818]
European Journal of Surgical Oncology Evaluation of serum lysyl oxidase as a blood test for colorectal cancer [Pubmed: 24246612]
Vaccine Copia autorizada por CDR [Ingentaconnect: Source]
Arthritis & Rheumatology Lysyl oxidase is a potential biomarker of fibrosis in systemic sclerosis [Pubmed: 24574233]
Eur J Surg Oncol.? Evaluation of serum lysyl oxidase as a blood test for colorectal cancer [Pubmed:Pmc4237001]
BMC Cancer. Ionizing radiation induces tumor cell lysyl oxidase secretion [Pubmed:Pmc4223762]
DNA Cell Biol. Lysyl Oxidase Genetic Variants Affect Gene Expression in Cervical Cancer [Pubmed:24945327]
scandinavian journal of immunology Increased Serum Levels of the IL-33 Neutralizing sST2 in Limited Cutaneous Systemic Sclerosis [PubMed: 26095613]
Curr Eye Res Correlation of Aqueous Humor Lysyl Oxidase Activity with TGF-ß Levels and LOXL1 Genotype in Pseudoexfoliation [Pubmed:27116380]
journal of bone and mineral metabolism LOX Fails to Substitute for RANKL in Osteoclastogenesis. [pubmed:27606829]
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