巨噬细胞炎性蛋白1α(MIP1a)活性蛋白

Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)

CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein

  • 巨噬细胞炎性蛋白1α(MIP1a)活性蛋白 产品包装(模拟)
  • 巨噬细胞炎性蛋白1α(MIP1a)活性蛋白 产品包装(模拟)
  • 巨噬细胞炎性蛋白1α(MIP1a)活性蛋白 Gene sequencing
  • APA092Hu61.jpg SDS-PAGE图
  • 巨噬细胞炎性蛋白1α(MIP1a)活性蛋白 Figure. Western Blot; Sample: APA092Hu61; Antibody: PAA092Hu06.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP1. Briefly, THP1 cells were seeded into the upper chambers (100µl cell suspension, 106 cells/ml in RPMI 1640 with 0.5% FBS) and MIP-1a (100ng/mL, diluted in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8µm pore size) used to separate the two compartments. After incubation at 37oC with 5% CO2 for 5h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×40) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).
By counting migrated cells in low chamber at high magnification (×400) randomly, it was shown that a mean of 41.2 THP1 cells/field migrated towards serum free RPMI 1640 medium with 100ng/mL MIP-1a, while only 3.6 THP1 cells/field migrated towards serum free RPMI 1640 medium. And the migrated THP1 cells in low chamber at low magnification (×40) was shown in Figure 1.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA092Hu01 巨噬细胞炎性蛋白1α(MIP1a)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA092Hu01 巨噬细胞炎性蛋白1α(MIP1a)活性蛋白 Cell culture; Activity Assays.
APA092Hu61 巨噬细胞炎性蛋白1α(MIP1a)活性蛋白 Cell culture; Activity Assays.
EPA092Hu61 巨噬细胞炎性蛋白1α(MIP1a)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA092Hu51 巨噬细胞炎性蛋白1α(MIP1a)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA092Hu07 巨噬细胞炎性蛋白1α(MIP1a)多克隆抗体 WB; IHC; ICC; IP.
PAA092Hu01 巨噬细胞炎性蛋白1α(MIP1a)多克隆抗体 WB; IHC; ICC; IP.
PAA092Hu06 巨噬细胞炎性蛋白1α(MIP1a)多克隆抗体 WB; IHC; ICC; IP.
MAA092Hu22 巨噬细胞炎性蛋白1α(MIP1a)单克隆抗体 WB; IHC; ICC; IP.
SEA092Hu 巨噬细胞炎性蛋白1α(MIP1a)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA092Hu 巨噬细胞炎性蛋白1α(MIP1a)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA092Hu01 巨噬细胞炎性蛋白1α(MIP1a)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".
PGA092Hu01 巨噬细胞炎性蛋白1α(MIP1a)引物对 PCR

参考文献

杂志 参考文献
Inhalation Toxicology Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans [Pubmed: 24044680]
Scientific Reports The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37Ra [Pubmed:26912204]
ActaMedicaMediterranea CIRCULATING MACROPHAGE INFLAMMATORY PROTEIN-1 ALPHA (MIP1-Α) AS A POTENTIAL BIOMARKER FOR THE DIAGNOSIS OF PATIENTS WITH … [57e97ac908aeb34bc08fd41a.pdf]
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