巨噬细胞移动抑制因子(MIF)活性蛋白

Active Macrophage Migration Inhibitory Factor (MIF)

GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

  • 巨噬细胞移动抑制因子(MIF)活性蛋白 产品包装(模拟)
  • 巨噬细胞移动抑制因子(MIF)活性蛋白 产品包装(模拟)
  • APA698Hu01.png Figure. SDS-PAGE
  • 巨噬细胞移动抑制因子(MIF)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Macrophage migration inhibitory factor (MIF), also known as glycosylation- inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MIF and recombinant human MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG- coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of MIF with MHCDG.

Macrophage migration inhibitory factor (MIF or MMIF), is a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. MIF contains two motifs with catalytic activity. The first is a 27 amino acid motif located at the N-terminus functions as a phenylpyruvate tautomerase. MIF also contains a Cys-Ala-Leu-Cys catalytic site between residues 57 and 60 that appears to function as a disulfide reductase. Besides, MIF is overexpressed in various tumors and has been suggested as a molecular link between chronic inflammation and cancer. To measured the effect of MIF on cell proliferation, breast cancer MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM prior to the addition of various concentrations of recombinant human MIF. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of MCF-7 cells after incubation with MIF for 96h observed by inverted microscope was shown in Figure 2. Cell viability was assessed by CCK-8 assay after incubation with recombinant MIF for 96h. The result was shown in Figure 3. It was obvious that MIF significantly increased cell viability of MCF-7 cells.
(A) MCF-7 cells cultured in DMEM, stimulated with 10ng/mL MIF for 96h;
(B) Unstimulated MCF7 cells cultured in DMEM for 96h.
Figure. Cell proliferation of MCF-7 cells after stimulated with MIF.

Figure. Cell proliferation of MCF-7 cells after stimulated with MIF.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA698Hu02 巨噬细胞移动抑制因子(MIF)活性蛋白 Cell culture; Activity Assays.
APA698Hu01 巨噬细胞移动抑制因子(MIF)活性蛋白 Cell culture; Activity Assays.
RPA698Hu02 巨噬细胞移动抑制因子(MIF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA698Hu01 巨噬细胞移动抑制因子(MIF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA698Hu01 巨噬细胞移动抑制因子(MIF)多克隆抗体 WB; IHC; ICC; IP.
PAA698Hu02 巨噬细胞移动抑制因子(MIF)多克隆抗体 WB,IHC
MAA698Hu22 巨噬细胞移动抑制因子(MIF)单克隆抗体 WB; IHC; ICC; IP.
SEA698Hu 巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA698Hu 巨噬细胞移动抑制因子(MIF)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA698Hu01 巨噬细胞移动抑制因子(MIF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Kidney International Targeted reduction of advanced glycation improves renal function in obesity [PubMed: 21412218]
The Journal of Immunology Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the Gut [Jimmunol: 5673]
The Journal of Endocrinology Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver disease [PubMed: PMC3757527]
The Journal of Immunology Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing mice [PubMed: PMC3466372]
Diabetologia Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetes [Pubmed:24957662]
BioMed Research International The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the Bladder [PubMed: 25705692]
Diabetologia Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease. [pubmed:28160092]
Life Sciences Protective effect of chlorogenic acid on the inflammatory damage of pancreas and lung in mice with l-arginine-induced pancreatitis [pubmed:28919396]
Scientific Reports Cytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic Stroke [Pubmed:29335619]
Ocular Immunology and Inflammation Immune Response and Mechanisms of IFN-γ in Administration for Keratomycosis [Pubmed: 30307777]
International Journal of Immunopathology and Pharmacology Effect of voluntary running on expression of myokines in brains of rats with depression [Pubmed: 30834799]
Biomarkers in Heart Failure and Associated Diseases Biomarkers in Heart Failure and Associated Diseases []
Science China-Life Sciences Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvation [Pubmed: 31209799]
Folia Morphologica Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expression [Pubmed: 31802473]
Biomed Pharmacother Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitis [Pubmed: 32062385]
Sci Rep Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population [33850223]
Commun Biol Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytes [Pubmed:35115654]
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