硫酸软骨素蛋白聚糖4(CSPG4)活性蛋白
Active Chondroitin Sulfate Proteoglycan 4 (CSPG4)
CSPG4; MSK16; HMW-MAA; MCSP; MCSPG; MEL-CSPG; NG2; Chondroitin Sulfate Proteoglycan 4; Melanoma chondroitin sulfate proteoglycan
- 编号APB134Hu02
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性状冻干粉
- 纯度> 97%
- 等电点6.4
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
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活性实验
Figure. The binding activity of MCSP with GAL8.
Melanoma-associated chondroitin sulfate proteoglycan (MCSP) is a chondroitin sulfate proteoglycan in humans. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. Besides, Galectin-8 (GAL8) has been identified as an interactor of MCSP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MCSP and recombinant human GAL8. Briefly, MCSP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GAL8-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MCSP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MCSP and GAL8 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. Western Blot
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPB134Hu02 | 硫酸软骨素蛋白聚糖4(CSPG4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB134Hu01 | 硫酸软骨素蛋白聚糖4(CSPG4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB134Hu02 | 硫酸软骨素蛋白聚糖4(CSPG4)活性蛋白 | Cell culture; Activity Assays. |
PAB134Hu01 | 硫酸软骨素蛋白聚糖4(CSPG4)多克隆抗体 | WB; IHC; ICC; IP. |
PAB134Hu02 | 硫酸软骨素蛋白聚糖4(CSPG4)多克隆抗体 | WB; IHC; ICC; IP. |
LAB134Hu71 | 硫酸软骨素蛋白聚糖4(CSPG4)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAB134Hu22 | 硫酸软骨素蛋白聚糖4(CSPG4)单克隆抗体 | WB; IHC; ICC; IP. |
SEB134Hu | 硫酸软骨素蛋白聚糖4(CSPG4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB134Hu | 硫酸软骨素蛋白聚糖4(CSPG4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
The Effect of Denture Settling Period before Inserting Immediately Loaded Implants Used for Assisting Mandibular Complete Overdenture: Metabolic Activity of Peri-Implant Tissues [] |