肌肉生长抑制素(MSTN)活性蛋白

Active Myostatin (MSTN)

GDF8; Growth Differentiation Factor 8

  • 肌肉生长抑制素(MSTN)活性蛋白 产品包装(模拟)
  • 肌肉生长抑制素(MSTN)活性蛋白 产品包装(模拟)
  • APB653Hu01.jpg SDS-PAGE图
  • 肌肉生长抑制素(MSTN)活性蛋白 Figure. Western Blot; Sample: Recombinant MSTN, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Myostatin (MSTN) also known as growth differentiation factor 8(GDF-8) is a member of the TGF beta protein family. Myostatin is aa secreted growth differentiation factor that produced and released by myocytes. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Besides, Bone Morphogenetic Protein 1 (BMP1) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MSTN and recombinant human BMP1. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of MSTN and BMP1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 10mM PBS to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APB653Hu01 肌肉生长抑制素(MSTN)活性蛋白 Cell culture; Activity Assays.
EPB653Hu61 肌肉生长抑制素(MSTN)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB653Hu01 肌肉生长抑制素(MSTN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB653Hu61 肌肉生长抑制素(MSTN)活性蛋白 Cell culture; Activity Assays.
CPB653Hu21 肌肉生长抑制素(MSTN)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAB653Hu08 肌肉生长抑制素(MSTN)多克隆抗体 WB; IHC; ICC; IP.
PAB653Hu06 肌肉生长抑制素(MSTN)多克隆抗体 WB; IHC; ICC; IP.
PAB653Hu01 肌肉生长抑制素(MSTN)多克隆抗体 Applications: WB; IHC; ICC; IP.
LAB653Hu71 肌肉生长抑制素(MSTN)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB653Hu22 肌肉生长抑制素(MSTN)单克隆抗体 WB; IHC; ICC; IP.
MAB653Hu21 肌肉生长抑制素(MSTN)单克隆抗体 WB; IHC; ICC; IP.
SEB653Hu 肌肉生长抑制素(MSTN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB653Hu 肌肉生长抑制素(MSTN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB653Hu 肌肉生长抑制素(MSTN)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSB653Hu11 肌肉生长抑制素(MSTN)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Clinical Medicine and Diagnostics Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized Rats [Sapub:Source]
Nutr Metab (Lond) Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice [PubMed: 26539241]
International Journal of Cancer Tumor inoculation site affects the development of cancer cachexia and muscle wasting [PubMed: 26016447]
Connect Tissue Res Induction of transient tenogenic phenotype of high density cultured human dermal fibroblasts [PubMed: 25748814]
PLoS One Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary Disease [Pubmed:26998756]
Calcif Tissue Int. The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats [Pubmed:27272029]
Archives of medical research Irisin and Myostatin Levels in Patients with Graves' Disease. [pubmed:27986127]
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