神经生长因子(NGF)活性蛋白

Active Nerve Growth Factor (NGF)

NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor

  • 神经生长因子(NGF)活性蛋白 产品包装(模拟)
  • 神经生长因子(NGF)活性蛋白 产品包装(模拟)
  • 神经生长因子(NGF)活性蛋白 Gene sequencing
  • APA105Hu01.jpg SDS-PAGE图
  • 神经生长因子(NGF)活性蛋白 Figure. Western Blot; Sample: APA105Hu01; Antibody: PAA105Hu01
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. As reported, when the pheochromocytoma cell line PC12 is exposed to nerve growth factor (NGF), the cells respond over a period of a week by ceasing cell division and extending neurites (Greene and Tischler, 1976). The cells were grown in Ham's F12K containing 5% fetal calf serum and 10% horse serum on  polylysine or collagen coated plates. When cells reached log phase growth , fresh medium was added together with 10ng/mL of NGF, then cells were observed by inverted microscope everyday.
Cell division ceasing and differentiation of PC12 cells after incubation with NGF (10ng/mL) for 6 days was shown in Figure1.Control group which received no NGF displayed no neurite outgrowth and cells multiply rapidly.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA105Hu01 神经生长因子(NGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA105Hu01 神经生长因子(NGF)活性蛋白 Cell culture; Activity Assays.
EPA105Hu61 神经生长因子(NGF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA105Hu01 神经生长因子(NGF)多克隆抗体 WB; IHC; ICC; IP.
PAA105Hu06 神经生长因子(NGF)多克隆抗体 WB; IHC; ICC; IP.
LAA105Hu71 神经生长因子(NGF)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA105Hu22 神经生长因子(NGF)单克隆抗体 WB; IHC; ICC; IP.
SEA105Hu 神经生长因子(NGF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA105Hu 神经生长因子(NGF)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA105Hu 神经生长因子(NGF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA105Hu01 神经生长因子(NGF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
The Tohoku Journal of Experimental Medicine Low-intensity aerobic exercise mitigates exercise-induced bronchoconstriction by improving the function of adrenal medullary chromaffin cells in asthmatic rats [Pubmed:25212248]
International Journal of Pharmaceutics Neurotrophic and antioxidant effects of silymarin comparable to 4-methylcatechol in protection against gentamicin-induced ototoxicity in guinea pigs [Pubmed:25445537]
Yonsei Medical Journal NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome [Pubmed:25510766]
Molecular and Cellular Endocrinology High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 [Pubmed:25614971]
Mol Cell Endocrinol High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 [PubMed: 25614971]
Neurosciences (Riyadh) Telmisartan inhibits hyperalgesia and inflammatory progression in a diabetic neuropathic pain model of Wistar rats. [PubMed: 25864063]
Neurotoxicity Research Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures [Pubmed:27189478]
Psychoneuroendocrinology Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism [Pubmed:26771945]
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064]
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