神经生长因子(NGF)活性蛋白

Active Nerve Growth Factor (NGF)

NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor

  • 神经生长因子(NGF)活性蛋白 产品包装(模拟)
  • 神经生长因子(NGF)活性蛋白 产品包装(模拟)
  • 神经生长因子(NGF)活性蛋白 Figure . Gene Sequencing (extract)
  • APA105Mu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Nerve Growth Factor (NGF) is the archetypal neurotrophin of a family of polypeptides .It has long occupied a critical role in developmental and adult neurobiology for its many important regulatory functions on the survival, growth and differentiation of nerve cells in the peripheral and central nervous system. The Caspase 3 (CASP3) is high affinity receptor for Nerve Growth Factor,thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse NGF and recombinant human CASP3. Briefly, NGF was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CASP3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse NGF and recombinant human CASP3 was shown in Figure 1, the EC50 for this effect is 0.86 ug/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
APA105Mu01 神经生长因子(NGF)活性蛋白 Cell culture; Activity Assays.
RPA105Mu01 神经生长因子(NGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA105Mu02 神经生长因子(NGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA105Mu03 神经生长因子(NGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA105Mu01 神经生长因子(NGF)多克隆抗体 WB; IHC; ICC; IP.
PAA105Mu02 神经生长因子(NGF)多克隆抗体 WB; IHC; ICC; IP.
MEA105Mu 神经生长因子(NGF)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA105Mu 神经生长因子(NGF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA105Mu 神经生长因子(NGF)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
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Yonsei Medical Journal NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome [Pubmed:25510766]
Molecular and Cellular Endocrinology High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 [Pubmed:25614971]
Mol Cell Endocrinol High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 [PubMed: 25614971]
Neurosciences (Riyadh) Telmisartan inhibits hyperalgesia and inflammatory progression in a diabetic neuropathic pain model of Wistar rats. [PubMed: 25864063]
Neurotoxicity Research Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures [Pubmed:27189478]
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MOLECULAR NUTRITION & FOOD RESEARCH Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and … [Pubmed: 32918393]
Neuropharmacology Impaired LTD-like motor cortical plasticity in female patients with major depression disorder [Pubmed: 32791084]
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BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways [33589896]
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