神经生长因子(NGF)活性蛋白

Active Nerve Growth Factor (NGF)

NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor

  • 神经生长因子(NGF)活性蛋白 产品包装(模拟)
  • 神经生长因子(NGF)活性蛋白 产品包装(模拟)
  • 神经生长因子(NGF)活性蛋白 Figure. Gene Sequencing (extract)
  • APA105Ra01.png Figure. SDS-PAGE
  • 神经生长因子(NGF)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. NGF is also involved in pathways besides those regulating the life cycle of neurons. a nerve growth factor (NGF)-dependent TF-1 subclone cell line suitable for determination of bioactivity of recombinant NGF(rhNGF), then TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 contain various concentrations of recombinant rat NGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of TF-1 cells after incubation with TF-1 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant NGF for 72h. The result was shown in Figure 2. It was obvious that NGF significantly increased cell viability of TF-1 cells.
(A) TF-1 cells cultured in 1640, stimulated with 0.1ng/mL NGF for 72h;
(B) Unstimulated TF-1 cells cultured in 1640 for 72h.
Figure. Cell proliferation of TF-1 cells after stimulated with NGF.

Figure. Cell proliferation of TF-1 cells after stimulated with NGF.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA105Ra01 神经生长因子(NGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA105Ra01 神经生长因子(NGF)活性蛋白 Cell culture; Activity Assays.
EPA105Ra61 神经生长因子(NGF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA105Ra01 神经生长因子(NGF)多克隆抗体 WB; IHC; ICC; IP.
MAA105Ra22 神经生长因子(NGF)单克隆抗体 WB; IHC; ICC; IP.
MAA105Ra21 神经生长因子(NGF)单克隆抗体 WB; IHC; ICC; IP.
SEA105Ra 神经生长因子(NGF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA105Ra 神经生长因子(NGF)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA105Ra 神经生长因子(NGF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA105Ra 神经生长因子(NGF)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA105Ra01 神经生长因子(NGF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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International Journal of Pharmaceutics Neurotrophic and antioxidant effects of silymarin comparable to 4-methylcatechol in protection against gentamicin-induced ototoxicity in guinea pigs [Pubmed:25445537]
Yonsei Medical Journal NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome [Pubmed:25510766]
Molecular and Cellular Endocrinology High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 [Pubmed:25614971]
Mol Cell Endocrinol High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 [PubMed: 25614971]
Neurosciences (Riyadh) Telmisartan inhibits hyperalgesia and inflammatory progression in a diabetic neuropathic pain model of Wistar rats. [PubMed: 25864063]
Neurotoxicity Research Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures [Pubmed:27189478]
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Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064]
The Journal of Maternal-Fetal & Neonatal Medicine Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities [pubmed:28081639]
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MOLECULAR NUTRITION & FOOD RESEARCH Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and … [Pubmed: 32918393]
Neuropharmacology Impaired LTD-like motor cortical plasticity in female patients with major depression disorder [Pubmed: 32791084]
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博士论文 Neurobiological determinants of depressive-like symptoms in rodents []
Medical Research Journal Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy []
Journal of the Taiwan Institute of Chemical Engineers Multiple-component dual-phase solid lipid nanoparticles with conjugated transferrin for formulating antioxidants and nerve growth factor against neuronal apoptosis []
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Cell Biochem Funct CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway [34296452]
Carbohydrate Polymers Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration [Pubmed:35550777]
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