摄食抑制因子1(NES1)活性蛋白

Active Nesfatin 1 (NES1)

  • 摄食抑制因子1(NES1)活性蛋白 产品包装(模拟)
  • 摄食抑制因子1(NES1)活性蛋白 产品包装(模拟)
  • APA242Ra01.png Figure. SDS-PAGE
  • 摄食抑制因子1(NES1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Nucb2 (Nucleobindin-2), a calcium-binding protein, is further cleaved into NES1 (Nesfatin-1). NES1 is an anorexigenic peptide and seems to participate in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. GADD45A (Growth arrest and DNA damage-inducible protein GADD45 alpha) has been identified as an interactor of Nucb2. Besides, Growth Arrest And DNA Damage Inducible Protein Beta (GADD45b) has been identified as an interactor of NES1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat NES1 and recombinant rat GADD45b. Briefly, NES1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to GADD45b-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NES1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NES1 and GADD45b was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of NES1 with GADD45b.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
EPA242Ra61 摄食抑制因子1(NES1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA242Ra01 摄食抑制因子1(NES1)活性蛋白 Cell culture; Activity Assays.
RPA242Ra01 摄食抑制因子1(NES1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA242Ra61 摄食抑制因子1(NES1)活性蛋白 Cell culture; Activity Assays.
PAA242Ra01 摄食抑制因子1(NES1)多克隆抗体 WB; IHC; ICC; IP.
LAA242Ra81 摄食抑制因子1(NES1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA242Ra71 摄食抑制因子1(NES1)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA242Ra 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA242Ra 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA242Ra 摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Bulletin of Clinical Psychopharmacology Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic Disorder [Psikofarmakoloji: 2043]
Progress in Neuro-Psychopharmacology & Biological Psychiatry High plasma nesfatin-1 level in patients with major depressive disorder [PubMed: 21163317]
Endocrine Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus [SpringerLink: h978088657555w16]
Bulletin of Clinical Psychopharmacology Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder [Psikofarmakoloji: 2212]
Psychoneuroendocrinology. Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder [PubMed: 22595767]
Iranian Journal of Health and Physical activity Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats [Ijvst: Source]
European Journal of Obstetrics & Gynecology and Reproductive Biology Decreased serum nesfatin-1 levels in endometriosis [Pubmed: 24702902]
Bulletin of Clinical Psychopharmacology Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder. [Psikofarmakoloji: Source]
Current Pharmaceutical Design Role of NUCB2/nesfatin-1 as a possible biomarker. [Pubmed: 23537082]
Regulatory, Integrative and Comparative Physiology A role of nesfatin-1/NucB2 in dehydration-induced anorexia [Pubmed:24829503]
J Endocrinol Invest. Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome [Pubmed:24920281]
Int J Surg. The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study. [Pubmed:25448651]
Hormone molecular biology and clinical investigation The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers [Pubmed:25581765]
Turk J Gastroenterol. New hormones to predict the severity of gallstone-induced acute pancreatitis [Pubmed:25599787]
Respiration Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1? [PubMed: 26088934]
J Clin Res Pediatr Endocninol Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children [PubMed: 26831553]
Bosn J Basic Med Sci Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndrome [PubMed: 26295295]
Original Article / Orijinal Makale Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1 [Sayilar: 294]
Gynecol Endocrinol Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome [PubMed: 26062107]
International Journal of Neuroscience Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage* [Pubmed:26872406]
未找到 Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairment [P.1.e.012.pdf]
International Journal of Developmental Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage. [pubmed:26872406]
Current Pharmaceutical Design Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease [pubmed:28137218]
Arch Endocrinol Metab Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes [pubmed:28977160]
Indian Journal of Psychiatry Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving? [pubmed:28529367]
Biomedicine & Pharmacotherapy Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ [Pubmed:29604590]
Biochimica et Biophysica Acta Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodeling [Pubmed:29627363]
BMC Psychiatry Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patients [Pubmed:29615007]
Probiotics and Antimicrobial Proteins Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar [Pubmed: 30232744]
An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages []
clinical neurology and neurosurgery Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels [Pubmed: 32311618]
Ann Nutr Metab Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity [Pubmed: 33027789]
Endokrynologia Polska Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke [33283259]
Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder []
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