Active Neutrophil Activating Protein 3 (NAP3)
CXCL1; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 97%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1814 ¥ 4536 ¥ 9072 ¥ 27216 ¥ 68040
- Figure. SDS-PAGE
- Figure. Western Blot
- 通过ISO 9001、ISO 13485质量体系认证
Figure. The chemotactic effect of NAP3 on THP-1 cells.
Neutrophil-activating protein 3 (NAP3) also known as chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family. NAP3 is expressed by macrophages, neutrophils and epithelial cells,and has neutrophil chemoattractant activity. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of NAP3 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension, 106 cells/mL in RPMI 1640 with FBS free) and SLC (31.25ng/mL, 62.5ng/mL ,125ng/mL and 250ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8µm pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows NAP3 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of NAP3 occurs at 31.25-250ng/mL.
(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 62.5ng/mL NAP3 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h;
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without NAP3 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h.
Figure.The chemotactic effect of NAP3 on THP-1 cells.
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|APA041Hu01||中性粒细胞激活蛋白3(NAP3)活性蛋白||Cell culture; Activity Assays.|
|RPA041Hu01||中性粒细胞激活蛋白3(NAP3)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA041Hu01||中性粒细胞激活蛋白3(NAP3)多克隆抗体||WB; ICC; IHC-P; IHC-F; ELISA.|
|LAA041Hu71||中性粒细胞激活蛋白3(NAP3)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|MAA041Hu22||中性粒细胞激活蛋白3(NAP3)单克隆抗体||WB; IHC; ICC; IP.|
|SEA041Hu||中性粒细胞激活蛋白3(NAP3)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA041Hu||中性粒细胞激活蛋白3(NAP3)检测试剂盒(化学发光免疫分析法)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA041Hu01||中性粒细胞激活蛋白3(NAP3)检测试剂盒DIY材料(酶联免疫吸附试验法)||Main materials for "Do It (ELISA Kit) Yourself".|
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|Research in Veterinary Science||A chimeric protein composed of the binding domains ofClostridium perfringens phospholipase C and Trueperella pyogenes pyolysin induces partial immunoprotection in a mouse model [Pubmed:27473983]|
|Scientific Reports||Epidermal CD147 expression plays a key role in IL-22-induced psoriatic dermatitis. [pubmed:28272440]|