诱导型一氧化氮合酶(NOS2)活性蛋白

Active Nitric Oxide Synthase 2, Inducible (NOS2)

NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2

  • 诱导型一氧化氮合酶(NOS2)活性蛋白 产品包装(模拟)
  • 诱导型一氧化氮合酶(NOS2)活性蛋白 产品包装(模拟)
  • APA837Mu01.jpg Figure. SDS-PAGE
  • 诱导型一氧化氮合酶(NOS2)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of NOS2 with UCHL5.
Nitric oxide synthase 2, inducible (NOS2) is a member of Nitric oxide synthases (NOSs) family. Nitric oxide synthases (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule. It helps modulate vascular tone, insulin secretion, airway tone, and peristalsis, and is involved in angiogenesis and neural development. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of NOS2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse NOS2 and recombinant mouse UCHL5. Briefly, NOS2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NOS2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NOS2 and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA837Mu03 诱导型一氧化氮合酶(NOS2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA837Mu01 诱导型一氧化氮合酶(NOS2)活性蛋白 Cell culture; Activity Assays.
RPA837Mu01 诱导型一氧化氮合酶(NOS2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA837Mu02 诱导型一氧化氮合酶(NOS2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA837Mu01 诱导型一氧化氮合酶(NOS2)多克隆抗体 WB; IHC; ICC; IP.
PAA837Mu02 诱导型一氧化氮合酶(NOS2)多克隆抗体 WB; IHC; ICC; IP.
PAA837Mu03 诱导型一氧化氮合酶(NOS2)多克隆抗体 WB; IHC; ICC; IP.
LAA837Mu71 诱导型一氧化氮合酶(NOS2)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA837Mu 诱导型一氧化氮合酶(NOS2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA837Mu 诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA837Mu01 诱导型一氧化氮合酶(NOS2)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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