头蛋白(NOG)活性蛋白

Active Noggin (NOG)

SYM1; SYNS1; Synostoses(Multiple)Syndrome 1; Symphalangism 1(Proximal)

  • 头蛋白(NOG)活性蛋白 产品包装(模拟)
  • 头蛋白(NOG)活性蛋白 产品包装(模拟)
  • 头蛋白(NOG)活性蛋白 Figure. Gene Sequencing (Extract)
  • APC130Hu01.png Figure. SDS-PAGE
  • 头蛋白(NOG)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of NOG with CDF5.
Noggin, also known as NOG, is a protein that is involved in the development of many body tissues, including nerve tissue, muscles, and bones. Noggin is a signaling molecule that plays an important role in promoting somite patterning in the developing embryo. It is released from the notochord and regulates bone morphogenic protein (BMP4) during development. It also causes formation of the head and other dorsal structures. Besides, Growth Differentiation Factor 5 (GDF5) has been identified as an interactor of NOG, thus a binding ELISA assay was conducted to detect the interaction of recombinant human NOG and recombinant human CDF5. Briefly, NOG were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CDF5-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NOG and CDF5 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APC130Hu01 头蛋白(NOG)活性蛋白 Cell culture; Activity Assays.
RPC130Hu01 头蛋白(NOG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC130Hu02 头蛋白(NOG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC130Hu01 头蛋白(NOG)多克隆抗体 WB; IHC; ICC; IP.
PAC130Hu02 头蛋白(NOG)多克隆抗体 WB; IHC; ICC; IP.
LAC130Hu71 头蛋白(NOG)多克隆抗体(生物素标记) WB; IHC; ICC.
LAC130Hu81 头蛋白(NOG)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAC130Hu22 头蛋白(NOG)单克隆抗体 WB; IHC; ICC; IP.
MAC130Hu24 头蛋白(NOG)单克隆抗体 WB; IHC; ICC; IP.
MAC130Hu26 头蛋白(NOG)单克隆抗体 WB; IHC; ICC; IP.
LAC130Hu76 头蛋白(NOG)单克隆抗体(生物素标记) WB; IHC; ICC.
SEC130Hu 头蛋白(NOG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
PSC130Hu01 头蛋白(NOG)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSC130Hu01 头蛋白(NOG)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Arthritis Rheumatol Imbalance between BMP2 and Noggin induces abnormal osteogenic differentiation of mesenchymal stem cells in ankylosing spondylitis [PubMed: 26413886]
Arthritis Rheumatol.  Imbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal Osteogenic Differentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis [Pubmed:26413886]
Clin Exp Pharmacol Physiol. Oxygen-glucose deprivation preconditioning protects neurons against oxygen-glucose deprivation/reperfusion induced injury via bone morphogenetic protein-7 mediated ERK, p38 and Smad signalling pathways. [Pubmed:26385023]
European cells & materials The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells. [pubmed:28266688]
Arthritis & Rheumatology mbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal OsteogenicDifferentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis. [pubmed:26413886]
Biotechnology and Applied Biochemistry The BMP antagonist Noggin is produced by osteoblasts in response to the presence of prostate cancer cells. [pubmed:28981962]
Heliyon Efficient in vitro delivery of Noggin siRNA enhances osteoblastogenesis [pubmed:29167826]
留言咨询