鸟氨酸脱羧酶(ODC)活性蛋白

Active Ornithine Decarboxylase (ODC)

ODC1

  • 鸟氨酸脱羧酶(ODC)活性蛋白 产品包装(模拟)
  • 鸟氨酸脱羧酶(ODC)活性蛋白 产品包装(模拟)
  • APB332Ra01.png Figure. SDS-PAGE
  • 鸟氨酸脱羧酶(ODC)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of ODC with TK1.
The enzyme ornithine decarboxylase (ODC) catalyzes the decarboxylation of ornithine (a product of the urea cycle) to form putrescine. This reaction is the committed step in polyamine synthesis. Lysine 69 on ornithine decarboxylase (ODC) binds the cofactor pyridoxal phosphate to form a Schiff base. Ornithine displaces the lysine to form a Schiff base attached to ODC, which decarboxylates to form a quinoid intermediate. This intermediate rearranges to form a Schiff base attached to putrescine, which is attacked by lysine to release putrescine product and reform PLP-bound ODC. Besides, Thymidine Kinase 1, Soluble (TK1) has been identified as an interactor of ODC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ODC and recombinant rat TK1. Briefly, ODC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TK1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ODC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ODC and TK1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB332Ra01 鸟氨酸脱羧酶(ODC)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB332Ra01 鸟氨酸脱羧酶(ODC)活性蛋白 Cell culture; Activity Assays.
PAB332Ra01 鸟氨酸脱羧酶(ODC)多克隆抗体 WB; IHC; ICC; IP.
MAB332Ra21 鸟氨酸脱羧酶(ODC)单克隆抗体 WB; IHC; ICC; IP.
SEB332Ra 鸟氨酸脱羧酶(ODC)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Biomedicine & Pharmacotherapy Insights on ornithine decarboxylase silencing as a potential strategy for targeting retinoblastoma [pubmed:29241071]
留言咨询