对氧磷酶1(PON1)活性蛋白

Active Paraoxonase 1 (PON1)

ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1

  • 对氧磷酶1(PON1)活性蛋白 产品包装(模拟)
  • 对氧磷酶1(PON1)活性蛋白 产品包装(模拟)
  • 对氧磷酶1(PON1)活性蛋白 Gene sequencing
  • APA243Hu01.jpg SDS-PAGE图
  • 对氧磷酶1(PON1)活性蛋白 Figure. Western Blot; Sample: Recombinant PON1, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Paraoxonase 1 (PON1) is responsible for hydrolysing organophosphate pesticides and nerve gasses. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). Besides, Clusterin (CLU) has been identified as an interactor of PON1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON1 and recombinant human CLU. Briefly, PON1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CLU-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PON1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of PON1 and CLU was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPA243Hu02 对氧磷酶1(PON1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA243Hu01 对氧磷酶1(PON1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA243Hu01 对氧磷酶1(PON1)活性蛋白 Cell culture; Activity Assays.
RPA243Hu03 对氧磷酶1(PON1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA243Hu04 对氧磷酶1(PON1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA243Hu05 对氧磷酶1(PON1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA243Hu01 对氧磷酶1(PON1)多克隆抗体 WB; IHC; ICC; IP.
PAA243Hu02 对氧磷酶1(PON1)多克隆抗体 WB; IHC; ICC; IP.
LAA243Hu71 对氧磷酶1(PON1)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA243Hu81 对氧磷酶1(PON1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA243Hu22 对氧磷酶1(PON1)单克隆抗体 WB; IHC; ICC; IP.
SEA243Hu 对氧磷酶1(PON1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA243Hu 对氧磷酶1(PON1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA243Hu 对氧磷酶1(PON1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA243Hu01 对氧磷酶1(PON1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Metabolic brain disease Individuals with autism have higher 8-Iso-PGF2α levels than controls, but no correlation with quantitative assay of Paraoxonase 1 serum levels [pubmed:28808839]
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GLYCOCONJUGATE JOURNAL Identification a novel clinical biomarker in early diagnosis of human non-small cell lung cancer [Pubmed: 30607521]
Hematology. Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktails [Pubmed: 30661467]
Proteomics Clinical Applications Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy [Pubmed: 31162828]
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Nutrition, Metabolism and Cardiovascular Diseases Phenotypes and concentration of PON1 in cardiovascular disease: the role of nutrient intake [Pubmed: 31757567]
Environment International Estimating renal and hepatic clearance rates of organophosphate esters in humans: Impacts of intrinsic metabolism and binding affinity with plasma proteins [Pubmed: 31783242]
RESPIRATORY RESEARCH The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation … [Pubmed: 33148285]
CLINICAL RHEUMATOLOGY PON-1 haplotype (-108C> T, L55M, and Q192R) modulates the serum levels and activity PONase promoting an atherogenic lipid profile in rheumatoid arthritis patients [Pubmed: 32556934]
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