甲状旁腺激素(PTH)活性蛋白

Active Parathyroid Hormone (PTH)

iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin

  • 甲状旁腺激素(PTH)活性蛋白 产品包装(模拟)
  • 甲状旁腺激素(PTH)活性蛋白 产品包装(模拟)
  • APA866Ra01.jpg SDS-PAGE图
  • 甲状旁腺激素(PTH)活性蛋白 Figure. Western Blot; Sample: Recombinant PTH, Rat.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

PTH (Parathyroid hormone) is a hormone secreted by the parathyroid glands that is important in bone remodeling. As reported, osteoblast-like cell lines, such as ROS 17/2.8, UMR106, SaOS, U2OS, MG63, that exhibit PTHR1, respond with increased proliferation to PTH. Rat PTH shares similarities with human PTH in amino acid sequence with the identity of 71.3%. Thus, a proliferation assay of rat recombinant PTH was conducted using U2OS cells. Briefly, U2OS cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of PTH. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of U2OS cells after incubation with PTH for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant PTH for 48h. The result was shown in Figure 2. It was obvious that PTH increased cell viability of U2OS cells.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APA866Ra01 甲状旁腺激素(PTH)活性蛋白 Cell culture; Activity Assays.
RPA866Ra01 甲状旁腺激素(PTH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA866Ra11 甲状旁腺激素(PTH)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA866Ra21 甲状旁腺激素(PTH)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA866Ra08 甲状旁腺激素(PTH)多克隆抗体 WB; IHC; ICC; IP.
PAA866Ra01 甲状旁腺激素(PTH)多克隆抗体 WB; IHC; ICC; IP.
MAA866Ra21 甲状旁腺激素(PTH)单克隆抗体 WB; IHC; ICC; IP.
CEA866Ra 甲状旁腺激素(PTH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA866Ra 甲状旁腺激素(PTH)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA866Ra 甲状旁腺激素(PTH)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA866Ra11 甲状旁腺激素(PTH)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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PREVENTIVE AND/OR THERAPEUTIC AGENT FOR OSTEOGENESIS IMPERFECTA AND OTHER DISEASES []
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Cells Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury [Pubmed:35456008]
African Health Sciences Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease []
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