血小板衍生生长因子A(PDGFA)活性蛋白

Active Platelet Derived Growth Factor Subunit A (PDGFA)

PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide

  • 血小板衍生生长因子A(PDGFA)活性蛋白 产品包装(模拟)
  • 血小板衍生生长因子A(PDGFA)活性蛋白 产品包装(模拟)
  • APA528Mu01.jpg SDS-PAGE图
  • 血小板衍生生长因子A(PDGFA)活性蛋白 Figure. Western Blot; Sample: Recombinant PDGFA, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

PDGFA (Platelet-derived growth factor subunit A) is a Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA has been described as a chemoattractant for monocytes and proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of PDGFA on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and PDGFA (1.5ng/mL, 7.5ng/mL and 15ng/mL diluted separately in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8µm pore size) used to separate the two compartments. After incubation at 37oC with 5%CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×40) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows PDGFA is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×40) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of PDGFA occurs at 1.5ng/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA528Mu01 血小板衍生生长因子A(PDGFA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA528Mu02 血小板衍生生长因子A(PDGFA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA528Mu01 血小板衍生生长因子A(PDGFA)活性蛋白 Cell culture; Activity Assays.
PAA528Mu01 血小板衍生生长因子A(PDGFA)多克隆抗体 WB; IHC; ICC; IP.
PAA528Mu02 血小板衍生生长因子A(PDGFA)多克隆抗体 WB; IHC; ICC; IP.
SEA528Mu 血小板衍生生长因子A(PDGFA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Canadian Journal of Physiology and Pharmacology The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680]
International Immunopharmacology Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway [pubmed:28521243]
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