M2-型丙酮酸激酶(PKM2)活性蛋白

Active Pyruvate kinase isozymes M2 (PKM2)

M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein; Pyruvate Kinase, Muscle

  • M2-型丙酮酸激酶(PKM2)活性蛋白 产品包装(模拟)
  • M2-型丙酮酸激酶(PKM2)活性蛋白 产品包装(模拟)
  • APA588Hu02.png Figure. SDS-PAGE
  • M2-型丙酮酸激酶(PKM2)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of PKM2 with PIN1.
Pyruvate Kinase, Muscle (PKM2) is one of four isozymes of pyruvate kinase. In vertebrates there are four isozymes of pyruvate kinase: L (liver), R (erythrocytes), M1 (muscles, hearts and brain) and M2 (only form detectable in early fetal tissue and present in most adult tissues). Pyruvate kinase is the enzyme that catalyzes the final step of glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP), yielding one molecule of pyruvate and one molecule of ATP. Besides, Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) has been identified as an interactor of PKM2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PKM2 and recombinant human PIN1. Briefly, PKM2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µL were then transferred to PIN1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PKM2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of PKM2 and PIN1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA588Hu01 M2-型丙酮酸激酶(PKM2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA588Hu02 M2-型丙酮酸激酶(PKM2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA588Hu03 M2-型丙酮酸激酶(PKM2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA588Hu02 M2-型丙酮酸激酶(PKM2)活性蛋白 Cell culture; Activity Assays.
PAA588Hu02 M2-型丙酮酸激酶(PKM2)多克隆抗体 WB; IHC; IF;FCM
PAA588Hu03 M2-型丙酮酸激酶(PKM2)多克隆抗体 WB; IHC; ICC; IP.
PAA588Hu01 M2-型丙酮酸激酶(PKM2)多克隆抗体 WB,IHC,ICC/IF
FAA588Hu01 抗M2-型丙酮酸激酶(PKM2)多克隆抗体 Flow cytometry.
FAA588Hu03 抗M2-型丙酮酸激酶(PKM2)多克隆抗体 Flow cytometry.
MAA588Hu22 M2-型丙酮酸激酶(PKM2)单克隆抗体 WB; IHC; ICC; IP.
MAA588Hu23 M2-型丙酮酸激酶(PKM2)单克隆抗体 WB; IHC; ICC; IP.
MAA588Hu21 M2-型丙酮酸激酶(PKM2)单克隆抗体 WB; IHC; ICC; IP.
SEA588Hu M2-型丙酮酸激酶(PKM2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA588Hu M2-型丙酮酸激酶(PKM2)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Cancer Research Antigen Presentation by Dendritic Cells in Tumors Is Disrupted by Altered Metabolism that Involves Pyruvate Kinase M2 and Its Interaction with SOCS3 [AACR: 70189]
Molecular BioSystems Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer [Pubmed:25483567]
Tumour Biol M2 isoform of pyruvate kinase (PKM2) is upregulated in Kazakh’s ESCC and promotes proliferation and migration of ESCC cells [PubMed: 26404132]
Oncotarget Identification of a serum biomarker panel for the differential diagnosis of cholangiocarcinoma and primary sclerosing cholangitis [Pubmed:29707118]
Journal of Neurotrauma Acteoside improves muscle atrophy and motor function by inducing new myokine secretion in chronic spinal cord injury [Pubmed: 30318996]
Cell Reports Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway [Pubmed: 32049010]
Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC) []
Cancer Cell International Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins []
Cancer Cell Int Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212]
Life (Basel) Putative Association between Low Baseline Gene Expression in the Peripheral Blood and Clinical Remission in Rheumatoid Arthritis Patients Treated with Tofacitinib [34947916]
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