再生胰岛衍生蛋白3β(REG3b)活性蛋白

Active Regenerating Islet Derived Protein 3 Beta (REG3b)

PAP1; Pancreatitis-Associated Protein 1

  • 再生胰岛衍生蛋白3β(REG3b)活性蛋白 产品包装(模拟)
  • 再生胰岛衍生蛋白3β(REG3b)活性蛋白 产品包装(模拟)
  • APK541Mu01.jpg SDS-PAGE图
  • 再生胰岛衍生蛋白3β(REG3b)活性蛋白 Figure. Western Blot; Sample: Recombinant REG3b, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Regenerating Islet Derived Protein 3 Beta (REG3b) also known as PAP-I and HIP is bactericidal C-type lectin which acts against several intestinal Gram-positive bacteria and Gram-negative bacteria. The Reg family proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. To test the effect of REG3b on cell proliferation of SK-N-SH, SK-N-SH cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of REG3b. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C .Cell proliferation of SK-N-SH cells after incubation with REG3b for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of REG3b was shown in Figure 2. It was obvious that REG3b significantly promoted cell proliferation of SK-N-SH cells. The ED50 for this effect is typically3.685-84.37ng/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
APK541Mu01 再生胰岛衍生蛋白3β(REG3b)活性蛋白 Cell culture; Activity Assays.
RPK541Mu01 再生胰岛衍生蛋白3β(REG3b)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPK541Mu02 再生胰岛衍生蛋白3β(REG3b)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAK541Mu01 再生胰岛衍生蛋白3β(REG3b)多克隆抗体 WB; IHC; ICC; IP.
PAK541Mu02 再生胰岛衍生蛋白3β(REG3b)多克隆抗体 WB; IHC; ICC; IP.
SEK541Mu 再生胰岛衍生蛋白3β(REG3b)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
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