Active Semaphorin 3A (SEMA3A)
SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 90%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 1g (保证有货 万一缺货 免费重做)
- 价格 ¥ 1872 ¥ 4680 ¥ 9360 ¥ 28080 ¥ 702000
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant SEMA3A, Human.
- 通过ISO 9001、ISO 13485质量体系认证
The Semaphorin 3A (SEMA3A) which belongs to the semaphorin family can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Semaphorin 3A is secreted protein containing a Sema domain, an immunoglobulin C2-like domain and a basic domain near the carboxyl tail. It can be secreted by neurons and surrounding tissue to guide migrating cells and axons in the developing nervous system. Besides, Neuropilin 1 (NRP1) has been identified as an interactor of SEMA3A, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SEMA3A and recombinant human NRP1. Briefly, SEMA3A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to NRP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SEMA3A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SEMA3A and NRP1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用|
|APL917Hu02||信号素3A(SEMA3A)活性蛋白||Cell culture; Activity Assays.|
|RPL917Hu01||信号素3A(SEMA3A)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPL917Hu02||信号素3A(SEMA3A)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAL917Hu01||信号素3A(SEMA3A)多克隆抗体||WB; IHC; ICC; IP.|
|PAL917Hu02||信号素3A(SEMA3A)多克隆抗体||WB; IHC; ICC; IP.|
|LAL917Hu71||信号素3A(SEMA3A)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|MAL917Hu21||信号素3A(SEMA3A)单克隆抗体||WB; IHC; ICC; IP.|
|LAL917Hu72||信号素3A(SEMA3A)单克隆抗体(生物素标记)||WB; IHC; ICC.|
|SEL917Hu||信号素3A(SEMA3A)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCL917Hu||信号素3A(SEMA3A)检测试剂盒(化学发光免疫分析法)||Chemiluminescent immunoassay for Antigen Detection.|
|Arthritis Research & Therapy||Semaphorin 3A is a marker for disease activity and a potential immunoregulator in systemic lupus erythematosus [Biomed: ar3881]|
|journal of invastigative dermatology||Cathelicidin LL-37 Induces Semaphorin 3A Expression in Human Epidermal Keratinocytes: Implications for Possible Application to Pruritus [PubMed: 26121211]|
|Neurology||Neurofilament light chain level is a weak risk factor for the development of MS. [pubmed:27521440]|