信号素3A(SEMA3A)活性蛋白

Active Semaphorin 3A (SEMA3A)

SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A

仅供体外研究使用,不用于临床诊断!警防假冒,可索取进口关税税单及报关单!
  • 信号素3A(SEMA3A)活性蛋白 产品包装(模拟)
  • 信号素3A(SEMA3A)活性蛋白 产品包装(模拟)
  • 信号素3A(SEMA3A)活性蛋白 Gene sequencing
  • APL917Hu02.jpg SDS-PAGE图
  • 信号素3A(SEMA3A)活性蛋白 Figure. Western Blot; Sample: Recombinant SEMA3A, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

The Semaphorin 3A (SEMA3A) which belongs to the semaphorin family can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Semaphorin 3A is secreted protein containing a Sema domain, an immunoglobulin C2-like domain and a basic domain near the carboxyl tail. It can be secreted by neurons and surrounding tissue to guide migrating cells and axons in the developing nervous system. Besides, Neuropilin 1 (NRP1) has been identified as an interactor of SEMA3A, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SEMA3A and recombinant human NRP1. Briefly, SEMA3A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to NRP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SEMA3A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SEMA3A and NRP1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用
APL917Hu02 信号素3A(SEMA3A)活性蛋白 Cell culture; Activity Assays.
RPL917Hu01 信号素3A(SEMA3A)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPL917Hu02 信号素3A(SEMA3A)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAL917Hu01 信号素3A(SEMA3A)多克隆抗体 WB; IHC; ICC; IP.
PAL917Hu02 信号素3A(SEMA3A)多克隆抗体 WB; IHC; ICC; IP.
LAL917Hu71 信号素3A(SEMA3A)多克隆抗体(生物素标记) WB; IHC; ICC.
MAL917Hu21 信号素3A(SEMA3A)单克隆抗体 WB; IHC; ICC; IP.
LAL917Hu72 信号素3A(SEMA3A)单克隆抗体(生物素标记) WB; IHC; ICC.
SEL917Hu 信号素3A(SEMA3A)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCL917Hu 信号素3A(SEMA3A)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

参考文献

杂志 参考文献
Arthritis Research & Therapy Semaphorin 3A is a marker for disease activity and a potential immunoregulator in systemic lupus erythematosus [Biomed: ar3881]
journal of invastigative dermatology Cathelicidin LL-37 Induces Semaphorin 3A Expression in Human Epidermal Keratinocytes: Implications for Possible Application to Pruritus [PubMed: 26121211]
Neurology Neurofilament light chain level is a weak risk factor for the development of MS. [pubmed:27521440]
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