性激素结合球蛋白(SHBG)活性蛋白

Active Sex Hormone Binding Globulin (SHBG)

ABP; TEBG; SBP; Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin

  • 性激素结合球蛋白(SHBG)活性蛋白 产品包装(模拟)
  • 性激素结合球蛋白(SHBG)活性蛋白 产品包装(模拟)
  • 性激素结合球蛋白(SHBG)活性蛋白 Figure . Gene Sequencing (extract)
  • APA396Hu61.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein, a molecule that consists of a carbohydrate plus a protein, and is produced mainly in your liver. It binds to three sex hormones found in both men and women: estrogen, dihydrotestosterone (DHT) and testosterone. Its job is to transport these hormones throughout your blood to other tissues in your body. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human SHBG and bsa-conjugated testosterone(Testo) . Briefly, biotin-linked recombinant human SHBG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to bsa-conjugated Testo -coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with HRP conjugage for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of SHBG and Testo was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
EPA396Hu61 性激素结合球蛋白(SHBG)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA396Hu62 性激素结合球蛋白(SHBG)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA396Hu02 性激素结合球蛋白(SHBG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA396Hu01 性激素结合球蛋白(SHBG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA396Hu63 性激素结合球蛋白(SHBG)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA396Hu61 性激素结合球蛋白(SHBG)活性蛋白 Cell culture; Activity Assays.
APA396Hu62 性激素结合球蛋白(SHBG)活性蛋白 Cell culture; Activity Assays.
PAA396Hu01 性激素结合球蛋白(SHBG)多克隆抗体 WB; IHC; ICC; IP.
PAA396Hu02 性激素结合球蛋白(SHBG)多克隆抗体 WB; IHC; ICC; IP.
LAA396Hu71 性激素结合球蛋白(SHBG)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA396Hu81 性激素结合球蛋白(SHBG)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA396Hu27 性激素结合球蛋白(SHBG)单克隆抗体 WB; IHC; ICC; IP.
MAA396Hu29 性激素结合球蛋白(SHBG)单克隆抗体 WB; IHC; ICC; IP.
MAA396Hu25 性激素结合球蛋白(SHBG)单克隆抗体 WB
MAA396Hu24 性激素结合球蛋白(SHBG)单克隆抗体 WB; IHC; ICC; IP.
MAA396Hu26 性激素结合球蛋白(SHBG)单克隆抗体 WB; IHC; ICC; IP.
MAA396Hu22 性激素结合球蛋白(SHBG)单克隆抗体 WB; IHC; ICC; IP.
MAA396Hu21 性激素结合球蛋白(SHBG)单克隆抗体 WB; IHC; ICC; IP.
MAA396Hu23 性激素结合球蛋白(SHBG)单克隆抗体 WB; IHC; ICC; IP.
SEA396Hu 性激素结合球蛋白(SHBG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA396Hu 性激素结合球蛋白(SHBG)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA396Hu01 性激素结合球蛋白(SHBG)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Nutrition and Cancer A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers [Tandfonline: Source]
international journal of environmental research and public health Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy [PubMed: 25996886]
Journal of Life Science Effects of Fermented Dandelion (Taraxacum coreanum) Extract on Male Climacteric Syndrome [SMGHBM_2016_v26n9_1063]
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373]
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389]
Toxicology and Applied Pharmacology Progressive effects of silver nanoparticles on hormonal regulation of reproduction in male rats [pubmed:27746313]
中国医药导报 益肾祛浊方对PCOS-IR大鼠Ghrelin、SHBG及VF的影响 [yycyzx201733003]
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146]
Biomed Pharmacother Comparative study on beneficial effects of vitamins B and D in attenuating doxorubicin induced cardiotoxicity in rats: Emphasis on calcium homeostasis [34029952]
Food Science and Biotechnology Improvement of testosterone deficiency by fermented Momordica charantia extracts in aging male rats [33868755]
Nutrients Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459]
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