Active Sirtuin 3 (SIRT3)
SIR2L3; Silent Mating Type Information Regulation 2 Homolog 3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; Regulatory protein SIR2 homolog 3
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 90%
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 1g (保证有货 万一缺货 免费重做)
- 价格 ¥ 1771 ¥ 4428 ¥ 8856 ¥ 26568 ¥ 664200
- Figure. Western Blot; Sample: Recombinant SIRT3, Mouse.
- 通过ISO 9001、ISO 13485质量体系认证
Sirtuin 3 (SIRT3) also known as NAD-dependent deacetylase sirtuin-3 is a member of the mammalian sirtuin family of proteins. SIRT3 is a soluble protein located in the mitochondrial matrix, and contains a mitochondrial processing peptide at the N-terminus. It is a key regulator of succinate dehydrogenase (SDH), which catalyzes the oxidation of succinate to fumarate. Increased succinate concentrations and the specific G protein-coupled receptor 91 (GPR91) are involved in the activation of hepatic stellate cells (HSCs) .SIRT3 agonists could help treat metabolic disorders such as obesity, metabolic syndrome and type 2 diabetes. Besides, Glutamate Dehydrogenase 1 (GDH) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SIRT3 and recombinant mouse GDH. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GDH-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SITR3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and GDH was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Mus musculus (Mouse，小鼠)||应用|
|APE913Mu01||沉默调节蛋白3(SIRT3)活性蛋白||Cell culture; Activity Assays.|
|RPE913Mu01||沉默调节蛋白3(SIRT3)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPE913Mu02||沉默调节蛋白3(SIRT3)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAE913Mu01||沉默调节蛋白3(SIRT3)多克隆抗体||WB; IHC; ICC; IP.|
|PAE913Mu02||沉默调节蛋白3(SIRT3)多克隆抗体||WB; IHC; ICC; IP.|
|SEE913Mu||沉默调节蛋白3(SIRT3)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|