沉默调节蛋白3(SIRT3)活性蛋白

Active Sirtuin 3 (SIRT3)

SIR2L3; Silent Mating Type Information Regulation 2 Homolog 3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; Regulatory protein SIR2 homolog 3

仅供体外研究使用,不用于临床诊断!警防假冒,可索取进口关税税单及报关单!
  • 沉默调节蛋白3(SIRT3)活性蛋白 产品包装(模拟)
  • 沉默调节蛋白3(SIRT3)活性蛋白 产品包装(模拟)
  • APE913Mu01.jpg SDS-PAGE图
  • 沉默调节蛋白3(SIRT3)活性蛋白 Figure. Western Blot; Sample: Recombinant SIRT3, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Sirtuin 3 (SIRT3) also known as NAD-dependent deacetylase sirtuin-3 is a member of the mammalian sirtuin family of proteins. SIRT3 is a soluble protein located in the mitochondrial matrix, and contains a mitochondrial processing peptide at the N-terminus. It is a key regulator of succinate dehydrogenase (SDH), which catalyzes the oxidation of succinate to fumarate. Increased succinate concentrations and the specific G protein-coupled receptor 91 (GPR91) are involved in the activation of hepatic stellate cells (HSCs) .SIRT3 agonists could help treat metabolic disorders such as obesity, metabolic syndrome and type 2 diabetes. Besides, Glutamate Dehydrogenase 1 (GDH) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SIRT3 and recombinant mouse GDH. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GDH-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SITR3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and GDH was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用
APE913Mu01 沉默调节蛋白3(SIRT3)活性蛋白 Cell culture; Activity Assays.
RPE913Mu01 沉默调节蛋白3(SIRT3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPE913Mu02 沉默调节蛋白3(SIRT3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAE913Mu01 沉默调节蛋白3(SIRT3)多克隆抗体 WB; IHC; ICC; IP.
PAE913Mu02 沉默调节蛋白3(SIRT3)多克隆抗体 WB; IHC; ICC; IP.
SEE913Mu 沉默调节蛋白3(SIRT3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
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