沉默调节蛋白3(SIRT3)活性蛋白

Active Sirtuin 3 (SIRT3)

SIR2L3; Silent Mating Type Information Regulation 2 Homolog 3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; Regulatory protein SIR2 homolog 3

  • 沉默调节蛋白3(SIRT3)活性蛋白 产品包装(模拟)
  • 沉默调节蛋白3(SIRT3)活性蛋白 产品包装(模拟)
  • APE913Ra01.jpg SDS-PAGE图
  • 沉默调节蛋白3(SIRT3)活性蛋白 Figure. Western Blot; Sample: Recombinant SIRT3, Rat.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Sirtuin 3 (SIRT3), the NAD-dependent deacetylaseis is member of the mammalian sirtuin family of proteins. In human, sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. It also can regulate epigenetic gene silencing and suppress recombination of rDNA in yeast. SIRT3 expression in white and brown adipose tissue. Besides, X-Ray Repair Cross Complementing 6 (XRCC6) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant Rat SIRT3 and recombinant Rat XRCC6. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to XRCC6-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SIRT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and XRCC6 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APE913Ra01 沉默调节蛋白3(SIRT3)活性蛋白 Cell culture; Activity Assays.
RPE913Ra01 沉默调节蛋白3(SIRT3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAE913Ra01 沉默调节蛋白3(SIRT3)多克隆抗体 WB; IHC; ICC; IP.
LAE913Ra71 沉默调节蛋白3(SIRT3)多克隆抗体(生物素标记) WB; IHC; ICC.
MAE913Ra21 沉默调节蛋白3(SIRT3)单克隆抗体 WB; IHC; ICC; IP.
SEE913Ra 沉默调节蛋白3(SIRT3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
留言咨询