Active Sirtuin 3 (SIRT3)
SIR2L3; Silent Mating Type Information Regulation 2 Homolog 3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; Regulatory protein SIR2 homolog 3
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1891 ¥ 4728 ¥ 9456 ¥ 28368 ¥ 70920
- Figure. SDS-PAGE
- Figure. Western Blot; Sample: Recombinant SIRT3, Rat.
- 通过ISO 9001、ISO 13485质量体系认证
Figure. The binding activity of SIRT3 with IDH2.
Sirtuin 3 (SIRT3), the NAD-dependent deacetylaseis is member of the mammalian sirtuin family of proteins. In human, sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. It also can regulate epigenetic gene silencing and suppress recombination of rDNA in yeast. SIRT3 expression in white and brown adipose tissue. Besides, Isocitrate Dehydrogenase 2, mitochondrial (IDH2) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SIRT3 and recombinant rat IDH2. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IDH2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SIRT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and IDH2 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Rattus norvegicus (Rat，大鼠)||应用(仅供研究使用，不用于临床诊断！)|
|APE913Ra01||沉默调节蛋白3(SIRT3)活性蛋白||Cell culture; Activity Assays.|
|RPE913Ra01||沉默调节蛋白3(SIRT3)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAE913Ra01||沉默调节蛋白3(SIRT3)多克隆抗体||WB; IHC; ICC; IP.|
|LAE913Ra71||沉默调节蛋白3(SIRT3)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|MAE913Ra21||沉默调节蛋白3(SIRT3)单克隆抗体||WB; IHC; ICC; IP.|
|SEE913Ra||沉默调节蛋白3(SIRT3)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|