干细胞因子(SCF)活性蛋白

Active Stem Cell Factor (SCF)

KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2

  • 干细胞因子(SCF)活性蛋白 产品包装(模拟)
  • 干细胞因子(SCF)活性蛋白 产品包装(模拟)
  • 干细胞因子(SCF)活性蛋白 Figure. Gene Sequencing (Extract)
  • APA120Mu01.png Figure. SDS-PAGE
  • 干细胞因子(SCF)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. Cell proliferation of Raw264.7 cells after stimulated with SCF.
Stem cell factor (also known as SCF, KIT-ligand, KL, or steel factor) is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. SCF has been shown to  stimulate the proliferation of Raw264.7 cells. To test this effect, Raw264.7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach later, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM containing various concentrations of recombinant mouse SCF. After incubated for 120h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of Raw264.7 cells after incubation with SCF for 120h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant SCF for 120h. The result was shown in Figure 2. It was obvious that SCF significantly increased cell viability of Raw264.7 cells.
(A) Raw264.7 cells cultured in DMEM, stimulated with 1ng/mL SCF for 120h;
(B) Unstimulated Raw264.7 cells cultured in DMEM for 120h.

Figure. Cell proliferation of Raw264.7 cells after stimulated with SCF.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA120Mu02 干细胞因子(SCF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA120Mu01 干细胞因子(SCF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA120Mu01 干细胞因子(SCF)活性蛋白 Cell culture; Activity Assays.
PAA120Mu02 干细胞因子(SCF)多克隆抗体 WB; IHC; ICC; IP.
PAA120Mu01 干细胞因子(SCF)多克隆抗体 WB; IHC; ICC; IP.
LAA120Mu81 干细胞因子(SCF)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA120Mu71 干细胞因子(SCF)多克隆抗体(生物素标记) WB; IHC; ICC.
MEA120Mu 干细胞因子(SCF)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA120Mu 干细胞因子(SCF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA120Mu 干细胞因子(SCF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

参考文献

杂志 参考文献
Archives of Gynecology and Obstetrics Effect of ovarian stimulation with recombinant follicle-stimulating hormone, gonadotropin-releasing hormone agonist and antagonists, on follicular fluid stem cell factor and serum urocortin 1 levels on the day of oocyte retrieval [Pubmed: 23801011]
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370]
Tumor Biology Hypoxia-induced secretion of IL-13 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]
Molecular Neurobiology Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology. [pubmed:27730513]
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