Active Stem Cell Factor (SCF)
KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2
- 物种Sus scrofa; Porcine (Pig，猪) 相同的名称，不同的物种。
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg (保证有货 万一缺货 免费重做)
- 价格 ¥ 2160 ¥ 5400 ¥ 10800 ¥ 32400 ¥ 81000
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant SCF, Porcine.
- 通过ISO 9001、ISO 13485质量体系认证
Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. SCF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 1x104cells/well and incubated for 72h in the presence or absence of various concentrations of SCF at 37oC. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.
Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1.
The dose-effect curve of SCF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 6.07 to 13.69ng/mL.
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Sus scrofa; Porcine (Pig，猪)||应用(仅供研究使用，不用于临床诊断！)|
|EPA120Po61||干细胞因子(SCF)真核蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA120Po61||干细胞因子(SCF)活性蛋白||Cell culture; Activity Assays.|
|PAA120Po06||干细胞因子(SCF)多克隆抗体||WB; IHC; ICC; IP.|
|MAA120Po21||干细胞因子(SCF)单克隆抗体||WB; IHC; ICC; IP.|
|SEA120Po||干细胞因子(SCF)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA120Po01||干细胞因子(SCF)检测试剂盒DIY材料(酶联免疫吸附试验法)||Main materials for "Do It (ELISA Kit) Yourself".|
|Archives of Gynecology and Obstetrics||Effect of ovarian stimulation with recombinant follicle-stimulating hormone, gonadotropin-releasing hormone agonist and antagonists, on follicular fluid stem cell factor and serum urocortin 1 levels on the day of oocyte retrieval [Pubmed: 23801011]|
|Medicine (Baltimore)||An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370]|
|Tumor Biology||Hypoxia-induced secretion of IL-13 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]|