Active Suppressors Of Cytokine Signaling 3 (SOCS3)
ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 97%
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1680 ¥ 4200 ¥ 8400 ¥ 25200 ¥ 63000
- Figure. Western Blot; Sample: Recombinant SOCS3, Mouse.
- 通过ISO 9001、ISO 13485质量体系认证
Suppressor of cytokine signaling 3 (SOCS3) is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family that negatively regulates cytokine signal transduction. SOCS3 is feedback inhibitors of the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) signaling pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. SOCS3 also can bind to JAK2 kinase inhibits its kinase activity and nhibits insulin signaling in adipose tissue and the liver. Besides, Glycoprotein 130 (gp130) has been identified as an interactor of SOCS3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SOCS3 and recombinant mouse gp130. Briefly, SOCS3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SOCS3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SOCS3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of SOCS3 and gp130 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Mus musculus (Mouse，小鼠)||应用(仅供研究使用，不用于临床诊断！)|
|RPB684Mu02||细胞因子信号转导抑制因子3(SOCS3)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB684Mu01||细胞因子信号转导抑制因子3(SOCS3)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APB684Mu01||细胞因子信号转导抑制因子3(SOCS3)活性蛋白||Cell culture; Activity Assays.|
|PAB684Mu02||细胞因子信号转导抑制因子3(SOCS3)多克隆抗体||WB; IHC; ICC; IP.|
|PAB684Mu01||细胞因子信号转导抑制因子3(SOCS3)多克隆抗体||WB; IHC; ICC; IP.|
|LAB684Mu71||细胞因子信号转导抑制因子3(SOCS3)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|LAB684Mu81||细胞因子信号转导抑制因子3(SOCS3)多克隆抗体(异硫氰酸荧光素标记)||WB; IHC; ICC; IF.|
|SEB684Mu||细胞因子信号转导抑制因子3(SOCS3)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|The Journal of Biological Chemistry||Human primary keratinocytes show a restricted ability to up-regulate suppressor of cytokine signaling (SOCS)3 compared to autologous macrophages [Jcb: 280370]|
|The Journal of Biological Chemistry||Human Primary Keratinocytes Show Restricted Ability to Up-regulate Suppressor of Cytokine Signaling (SOCS)3 Protein Compared with Autologous Macrophages [PubMed: PMC3323061]|
|Journal of Reproductive Immunology||What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? [Pubmed: 24368037]|
|The Journal of Immunology||Alveolar Epithelial Cell–Derived Prostaglandin E2 Serves as a Request Signal for Macrophage Secretion of Suppressor of Cytokine Signaling 3 during Innate Inflammation [Pubmed:27183597]|
|Scientific Reports||The FXR Agonist, Obeticholic Acid, Suppresses HCC Proliferation & Metastasis: Role of IL-6/STAT3 Signalling Pathway [pubmed:28970500]|
|Journal of Immunological Methods||Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs. [pubmed:29154772]|