表面活性物质关联蛋白C(SP-C)活性蛋白

Active Surfactant Protein C (SP-C)

SFTPC; PSP-C; SFTP2; SPC; SP5; Surfactant Associated Protein C; Pulmonary Surfactant Protein C; Pulmonary surfactant-associated proteolipid SPL(Val)

  • 表面活性物质关联蛋白C(SP-C)活性蛋白 产品包装(模拟)
  • 表面活性物质关联蛋白C(SP-C)活性蛋白 产品包装(模拟)
  • 表面活性物质关联蛋白C(SP-C)活性蛋白 Figure. Gene Sequencing (Extract)
  • APB623Ra01.jpg SDS-PAGE图
  • 表面活性物质关联蛋白C(SP-C)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Surfactant Associated Protein C (SPC) is one of the pulmonary surfactant proteins. It is a membrane protein which manufactures surfactant. The propeptide of pulmonary surfactant C has an N-terminal alpha-helical segment whose suggested function was stabilization of the protein structure, since the latter can irreversibly transform from its native alpha-helical structure to beta-sheet aggregates and form amyloid fibrils. Besides, Monokine Induced By Interferon Gamma (MIg) has been identified as an interactor of SPC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SPC and recombinant rat MIg. Briefly, SPC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to MIg-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SPC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SPC and MIg was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of SPC with MIg.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APB623Ra01 表面活性物质关联蛋白C(SP-C)活性蛋白 Cell culture; Activity Assays.
RPB623Ra01 表面活性物质关联蛋白C(SP-C)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB623Ra02 表面活性物质关联蛋白C(SP-C)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB623Ra01 表面活性物质关联蛋白C(SP-C)多克隆抗体 WB; IHC; ICC; IP.
PAB623Ra02 表面活性物质关联蛋白C(SP-C)多克隆抗体 WB; IHC; ICC; IP.
MAB623Ra21 表面活性物质关联蛋白C(SP-C)单克隆抗体 WB; IHC; ICC; IP.
SEB623Ra 表面活性物质关联蛋白C(SP-C)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB623Ra 表面活性物质关联蛋白C(SP-C)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
American Journal of Rhinology & Allergy Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps [PubMed: 23406594]
Plos one Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins [PubMed: PMC3551896]
Plos one The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS [PubMed: PMC3787032]
Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge [Opus4:Source]
Mol Cell Biochem Autophagy regulates hyperoxia-induced intracellular accumulation of surfactant protein C in alveolar type II cells [PubMed: 26122393]
Plos One Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) [Pubmed:27337142]
PLoS One The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions. [pubmed:27656877]
Frontiers in Aging Neuroscience Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid. [pubmed:28101052]
Frontiers in Aging Neuroscience Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid [PMC5209370]
American Journal of Physiology. Lung Cellular and Molecular Physiology Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. [pubmed:29167126]
Respiratory research Effects and molecular mechanisms of intrauterine infection/inflammation on lung development [Pubmed:29747649]
Lung  Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation [Pubmed:29445934]
Molecular Neurobiology Rheologically Essential Surfactant Proteins of the CSF Interacting with Periventricular White Matter Changes in Hydrocephalus Patients–Implications for CSF … [Doi: 10.1007/s12035-019-01648-z]
Stem Cells International Adipose Tissue-Derived Stem Cells Have the Ability to Differentiate into Alveolar Epithelial Cells and Ameliorate Lung Injury Caused by Elastase-Induced Emphysema … []
Stem Cells International Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor [Pubmed: 31949433]
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