可溶性胸苷激酶1(TK1)活性蛋白

Active Thymidine Kinase 1, Soluble (TK1)

TK2; Thymidine kinase, cytosolic

  • 可溶性胸苷激酶1(TK1)活性蛋白 产品包装(模拟)
  • 可溶性胸苷激酶1(TK1)活性蛋白 产品包装(模拟)
  • APC823Hu01.png Figure. SDS-PAGE
  • 可溶性胸苷激酶1(TK1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of TK1 with GAPDH.
Thymidine kinase 1 (TK1), is a human thymidine kinase. Thymidine kinase has been making a growing impact in the cancer research community. It has been found that elevated blood serum levels of TK-1 correlates with metastatic capabilities of the cancer and thereby can be used to detect malignant types of cancer, furthermore TK-1 has been found to show up in blood serum even before clinical symptoms even start to show. Besides, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) has been identified as an interactor of TK1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TK1 and recombinant human GAPDH. Briefly, TK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GAPDH-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TK1 and GAPDH was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPC823Hu01 可溶性胸苷激酶1(TK1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC823Hu01 可溶性胸苷激酶1(TK1)活性蛋白 Cell culture; Activity Assays.
PAC823Hu01 可溶性胸苷激酶1(TK1)多克隆抗体 WB
LAC823Hu71 可溶性胸苷激酶1(TK1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC823Hu22 可溶性胸苷激酶1(TK1)单克隆抗体 WB,ICC/IF
MAC823Hu21 可溶性胸苷激酶1(TK1)单克隆抗体 WB; IHC; ICC; IP.
MAC823Hu23 可溶性胸苷激酶1(TK1)单克隆抗体 WB
MAC823Hu24 可溶性胸苷激酶1(TK1)单克隆抗体 WB; IHC; ICC; IP.
MAC823Hu25 可溶性胸苷激酶1(TK1)单克隆抗体 WB; IHC; ICC; IP.
SEC823Hu 可溶性胸苷激酶1(TK1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC823Hu 可溶性胸苷激酶1(TK1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
PSC823Hu01 可溶性胸苷激酶1(TK1)抗体对 ELISA,CLIA,ELISPOT,Luminex
KSC823Hu01 可溶性胸苷激酶1(TK1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Mol Imaging Biol Evaluation of [18F] Fluorothymidine as a Biomarker for Early Therapy Response in a Mouse Model of Colorectal Cancer [Pubmed:27324368]
Molecular Imaging and Biology Evaluation of [18F]Fluorothymidine as a Biomarker for Early Therapy Response in a Mouse Model of Colorectal Cancer [pubmed:27324368]
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