组织金属蛋白酶抑制因子3(TIMP3)活性蛋白

Active Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)

SFD; TIMP Metallopeptidase Inhibitor 3; Metallopeptidase Inhibitor 3; Sorsby Fundus Dystrophy; Pseudoinflammatory

  • 组织金属蛋白酶抑制因子3(TIMP3)活性蛋白 产品包装(模拟)
  • 组织金属蛋白酶抑制因子3(TIMP3)活性蛋白 产品包装(模拟)
  • APA129Hu01.png Figure. SDS-PAGE
  • 组织金属蛋白酶抑制因子3(TIMP3)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) is an protein belongs to the tissue inhibitor of metalloproteinases family. They are inhibitors of the matrix metalloproteinases. TIMP-3 is the only member of the TIMP family which is found exclusively in the extracellular matrix (ECM). It is regulated in a cell cycle-dependent fashion in certain cell types and may serve as a marker for terminal differentiation. The activity of recombinant human TIMP3 was measured by its ability to inhibit recombinant human MMP2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (v/v), pH 7.5. The rhMMP-2 (APA100Hu61) was activated with 1 mM APMA at 37 °C for 1h. Then 16 μl 126 ug/mL rhTIMP-3, 25.6 µL of 100 ug/ml rhMMP-2 and 118.4 µL of assay buffer was incubated for 2 hours at 37 °C, including a control (in duplicate) containing assay buffer and the diluted rhMMP-2. The mixtures was diluted 5 fold in assay buffer followed by adding 50 ul 20 uM substrate, including a control containing assay buffer and substrate. Then read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively in kinetic mode for 5 minutes. Under these conditions, the enzyme amount of 50% inhibition of rhMMP-2 activity per minute is defined as a unit. The specific activity of TIMP3 is >6000 U/mg.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA129Hu01 组织金属蛋白酶抑制因子3(TIMP3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA129Hu01 组织金属蛋白酶抑制因子3(TIMP3)活性蛋白 Cell culture; Activity Assays.
PAA129Hu01 组织金属蛋白酶抑制因子3(TIMP3)多克隆抗体 WB; IHC; ICC; IP.
MAA129Hu21 组织金属蛋白酶抑制因子3(TIMP3)单克隆抗体 WB; IHC; ICC; IP.
MAA129Hu22 组织金属蛋白酶抑制因子3(TIMP3)单克隆抗体 WB; IHC; ICC; IP.
SEA129Hu 组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA129Hu 组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA129Hu 组织金属蛋白酶抑制因子3(TIMP3)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Neuroimmunomodulation Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus [Pubmed: source]
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells [Rsmjournals: Source]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Physiol. Res. The unwounded skin remodeling in animal models of diabetes types 1 and 2. [Pubmed: 24020818]
VirusDisease Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection [Pubmed:26925446]
Acta Pharmacol Sin. Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. [pubmed:27890915]
Biochemical genetics Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer [pubmed:28421310]
International Journal of Cancer Circulating and tissue IMP3 levels are correlated with poor survival in renal cell carcinoma [Pubmed: 30650187]
J. Pers. Med Altered TIMP-3 Levels in the Cerebrospinal Fluid and Plasma of Patients with Alzheimer's Disease [Pubmed:35629249]
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