转化生长因子β2(TGFb2)活性蛋白

Active Transforming Growth Factor Beta 2 (TGFb2)

TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor

  • 转化生长因子β2(TGFb2)活性蛋白 产品包装(模拟)
  • 转化生长因子β2(TGFb2)活性蛋白 产品包装(模拟)
  • APA218Hu01.png Figure. SDS-PAGE
  • 转化生长因子β2(TGFb2)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Transforming growth factor-beta 2 (TGFb2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. There are two named isoforms of this protein, created by alternative splicing of the same gene. Besides, Amyloid Precursor Protein (APP) has been identified as an interactor of TGFb2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TGFb2 and recombinant human APP. Briefly, TGFb2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APP-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TGFb2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TGFb2 and APP was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of TGFb2 with APP.

Transforming growth factor beta (TGF-β) is a multifunctional cytokine belonging to the transforming growth factor superfamily. The TGF-β superfamily includes endogenous growth inhibiting proteins; an increase in expression of TGF-β often correlates with the malignancy of many cancers and a defect in the cellular growth inhibition response to TGF-β. Its immunosuppressive functions then come to dominate, contributing to oncogenesis. To test the effect of TGF-β on inhibit HGF-dependent proliferation, HepG2 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1ng/mL HGF prior to the addition of various concentrations of recombinant human TGF-β. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. The inhibitory effect of TGF-β on HGF-dependent proliferation of HepG2 cells observed by inverted microscope was shown in Figure 2. Cell viability was assessed by CCK-8 assay after incubation with recombinant TGF-β for 96h. The result was shown in Figure 3. It was obvious that TGF-β significantly decreased cell viability of HepG2 cells. (A) HepG2 cells cultured in DMEM, stimulated with 1µg/mL TGF-β for 96h; (B) Unstimulated HepG2 cells cultured in DMEM for 96h.
Figure.The inhibitory effect of TGF-β on cell proliferation of HepG2 cells.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA218Hu01 转化生长因子β2(TGFb2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA218Hu01 转化生长因子β2(TGFb2)活性蛋白 Cell culture; Activity Assays.
PAA218Hu01 转化生长因子β2(TGFb2)多克隆抗体 WB; IHC
RAA218Hu21 转化生长因子β2(TGFb2)重组抗体 WB; IF; ICC; IHC; IP; FCM.
MAA218Hu21 转化生长因子β2(TGFb2)单克隆抗体 WB; IHC; ICC; IP.
MAA218Hu22 转化生长因子β2(TGFb2)单克隆抗体 WB; IHC; ICC; IP.
FAA218Hu08 抗转化生长因子β2(TGFb2)单克隆抗体 Flow cytometry.
FAA218Hu06 抗转化生长因子β2(TGFb2)单克隆抗体 Flow cytometry.
FAA218Hu04 抗转化生长因子β2(TGFb2)单克隆抗体 Flow cytometry.
FAA218Hu02 抗转化生长因子β2(TGFb2)单克隆抗体 Flow cytometry.
SEA218Hu 转化生长因子β2(TGFb2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA218Hu 转化生长因子β2(TGFb2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA218Hu 转化生长因子β2(TGFb2)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source]
J Reprod Immunol The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173]
International Journal of Chemical Studies Studies on quality attributes of skimmed colostrum powder [P-ISSN: 2349–8528]
JCI Insight Angiocrine signals regulate quiescence and therapy resistance in bone metastasis [Pubmed: 31292293]
Mol Med Association of CASC18/miR-20a-3p/TGFB2 ceRNA axis with occult lymph node metastasis in tongue squamous cell carcinoma [34362313]
Food Chem Effect of convection and microwave heating on the retention of bioactive components in human milk [34896952]
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