转化生长因子β3(TGFb3)活性蛋白

Active Transforming Growth Factor Beta 3 (TGFb3)

TGF-B3; LAP; Latency-associated peptide

  • 转化生长因子β3(TGFb3)活性蛋白 产品包装(模拟)
  • 转化生长因子β3(TGFb3)活性蛋白 产品包装(模拟)
  • APB949Hu01.png Figure. SDS-PAGE
  • 转化生长因子β3(TGFb3)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of TGFb3 with TGFbR2.
Transforming growth factor beta-3 (TGFb3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGFb3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. It also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. Besides, Transforming Growth Factor Beta Receptor II (TGFbR2) has been identified as an interactor of TGFb3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TGFb3 and recombinant human TGFbR2. Briefly, TGFb3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TGFbR2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TGFb3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TGFb3 and TGFbR2 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPB949Hu02 转化生长因子β3(TGFb3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB949Hu02 转化生长因子β3(TGFb3)活性蛋白 Cell culture; Activity Assays.
RPB949Hu01 转化生长因子β3(TGFb3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB949Hu01 转化生长因子β3(TGFb3)活性蛋白 Cell culture; Activity Assays.
PAB949Hu01 转化生长因子β3(TGFb3)多克隆抗体 WB; IHC; ICC; IP.
PAB949Hu02 转化生长因子β3(TGFb3)多克隆抗体 WB; IHC; ICC; IP.
LAB949Hu71 转化生长因子β3(TGFb3)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB949Hu23 转化生长因子β3(TGFb3)单克隆抗体 WB; IHC; ICC; IP.
RAB949Hu21 转化生长因子β3(TGFb3)重组抗体 WB; IF; ICC; IHC; IP; FCM.
MAB949Hu22 转化生长因子β3(TGFb3)单克隆抗体 WB
MAB949Hu24 转化生长因子β3(TGFb3)单克隆抗体 WB; IHC; ICC; IP.
MAB949Hu21 转化生长因子β3(TGFb3)单克隆抗体 WB; IHC; ICC; IP.
SEB949Hu 转化生长因子β3(TGFb3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB949Hu 转化生长因子β3(TGFb3)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB949Hu 转化生长因子β3(TGFb3)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
PSB949Hu01 转化生长因子β3(TGFb3)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB949Hu01 转化生长因子β3(TGFb3)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
J Reprod Immunol The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173]
Fertility and Sterility Influence of vitamin D and transforming growth factor β3 serum concentrations, obesity, and family history on the risk for uterine fibroids [pubmed:27743697]
Eur J Pharm Biopharm New scaffolds encapsulating TGF-β3/BMP-7 combinations driving strong chondrogenic differentiation [pubmed:28087378]
Transforme edici büyüme faktörü-beta-1 nötralizan antikoru ve transforme edici büyüme faktörü-beta-3'ün trakeal darlık gelişimi üzerine etkileri [10.5606/tgkdc.dergisi.2017.14302]
Fertility and Sterility Ulipristal acetate decreases transforming growth factor β3 serum and tumor tissue concentrations in patients with uterine fibroids [Pubmed:29525690]
Scientific Reports TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration [Pubmed:29795291]
Immunology Letters The involvement of multifunctional TGF-β and related cytokines in pathogenesis of endometriosis [Pubmed: 30367890]
Cell Reports Transforming Growth Factor-β3 Regulates Adipocyte Number in Subcutaneous White Adipose Tissue [Pubmed: 30332637]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Temporal TGF-β Supergene Family Signalling Cues Modulating Tissue Morphogenesis: Chondrogenesis within a Muscle Tissue Model? [Pubmed: 32660137]
Equine Vet J Limited added value of negative pressure wound therapy compared to calcium alginate dressings for second intention healing in a non©\contaminated and?¡­ [34115409]
AAPS PharmSciTech Nanoparticles and Nanostructured Films with TGF-β3: Preparation, Characterization, and Efficacy [34378118]
留言咨询