角质转谷氨酰胺酶(TGM1)活性蛋白

Active Transglutaminase 1, Keratinocyte (TGM1)

TGK; ICR2; KTG; LI; LI1; TGASE; Transglutaminase K; K Polypeptide Epidermal Type I; Protein-Glutamine-Gamma-Glutamyltransferase

  • 角质转谷氨酰胺酶(TGM1)活性蛋白 产品包装(模拟)
  • 角质转谷氨酰胺酶(TGM1)活性蛋白 产品包装(模拟)
  • APB773Mu01.png Figure. SDS-PAGE
  • 角质转谷氨酰胺酶(TGM1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of TGM1 with SNCa.
Transglutaminase 1(TGM1) is an enzyme found in cells that make up the outermost layer of the skin (the epidermis). It can catalyze the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. TGM1 is involved in the formation of the cornified cell envelope, which is a structure that surrounds skin cells and helps form a protective barrier between the body and its environment. Besides, Synuclein Alpha (SNCa) has been identified as an interactor of TGM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse TGM1 and recombinant mouse SNCa. Briefly, TGM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SNCa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TGM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TGM1 and SNCa was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB773Mu02 角质转谷氨酰胺酶(TGM1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB773Mu01 角质转谷氨酰胺酶(TGM1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB773Mu01 角质转谷氨酰胺酶(TGM1)活性蛋白 Cell culture; Activity Assays.
PAB773Mu01 角质转谷氨酰胺酶(TGM1)多克隆抗体 WB; IHC; ICC; IP.
PAB773Mu02 角质转谷氨酰胺酶(TGM1)多克隆抗体 WB; IHC; ICC; IP.
LAB773Mu81 角质转谷氨酰胺酶(TGM1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB773Mu71 角质转谷氨酰胺酶(TGM1)多克隆抗体(生物素标记) WB; IHC; ICC.
SEB773Mu 角质转谷氨酰胺酶(TGM1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
留言咨询