心肌肌钙蛋白I(cTnI)活性蛋白

Active Cardiac Troponin I (cTnI)

TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac

  • 心肌肌钙蛋白I(cTnI)活性蛋白 产品包装(模拟)
  • 心肌肌钙蛋白I(cTnI)活性蛋白 产品包装(模拟)
  • APA478Hu01.png Figure. SDS-PAGE
  • 心肌肌钙蛋白I(cTnI)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of TNNI3 with TNNT2.
Troponin I Type 3, Cardiac (TNNI3) is a protein that in humans is encoded by the TNNI3 gene. It is a tissue-specific subtype of troponin I, which in turn is a part of the troponin complex. The TNNI3 gene encoding cardiac troponin I (cTnI) is located at 19q13.4 in the human chromosomal genome. Human cTnI is a 24kDa protein consisting of 210 amino acids with isoelectric point (pI) of 9.87. cTnI is exclusively expressed in adult cardiac muscle. Besides, Troponin T Type 2, Cardiac (TNNT2) has been identified as an interactor of TNNI3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNNI3 and recombinant human TNNT2. Briefly, TNNI3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to TNNT2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TNNI3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNNI3 and TNNT2 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA478Hu02 心肌肌钙蛋白I(cTnI)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA478Hu61 心肌肌钙蛋白I(cTnI)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA478Hu01 心肌肌钙蛋白I(cTnI)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA478Hu01 心肌肌钙蛋白I(cTnI)活性蛋白 Cell culture; Activity Assays.
PAA478Hu01 心肌肌钙蛋白I(cTnI)多克隆抗体 WB; IHC; ICC; IP.
LAA478Hu71 心肌肌钙蛋白I(cTnI)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA478Hu28 心肌肌钙蛋白I(cTnI)单克隆抗体 WB; IHC; ICC; IP.
RAA478Hu21 心肌肌钙蛋白I(cTnI)重组抗体 WB; IHC; ICC.
MAA478Hu21 心肌肌钙蛋白I(cTnI)单克隆抗体 WB; IHC; ICC; IP.
MAA478Hu27 心肌肌钙蛋白I(cTnI)单克隆抗体 WB; IHC; ICC; IP.
MAA478Hu23 心肌肌钙蛋白I(cTnI)单克隆抗体 WB; IHC; ICC; IP.
MAA478Hu24 心肌肌钙蛋白I(cTnI)单克隆抗体 WB; IHC; ICC; IP.
MAA478Hu25 心肌肌钙蛋白I(cTnI)单克隆抗体 WB; IHC; ICC; IP.
MAA478Hu29 心肌肌钙蛋白I(cTnI)单克隆抗体 WB; IHC; ICC; IP.
MAA478Hu22 心肌肌钙蛋白I(cTnI)单克隆抗体 WB; IHC; ICC; IP.
MAA478Hu26 心肌肌钙蛋白I(cTnI)单克隆抗体 WB; IHC; ICC; IP.
FAA478Hu08 抗心肌肌钙蛋白I(cTnI)单克隆抗体 FCM
FAA478Hu06 抗心肌肌钙蛋白I(cTnI)单克隆抗体 Flow cytometry.
FAA478Hu04 抗心肌肌钙蛋白I(cTnI)单克隆抗体 Flow cytometry.
FAA478Hu02 抗心肌肌钙蛋白I(cTnI)单克隆抗体 Flow cytometry.
LAA478Hu74 心肌肌钙蛋白I(cTnI)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA478Hu 心肌肌钙蛋白I(cTnI)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA478Hu 心肌肌钙蛋白I(cTnI)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA478Hu 抗心肌肌钙蛋白I抗体(Anti-cTnI)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA478Hu 心肌肌钙蛋白I(cTnI)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA478Hu 心肌肌钙蛋白I(cTnI)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA478Hu01 心肌肌钙蛋白I(cTnI)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

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