肿瘤坏死因子β(TNFb)活性蛋白

Active Tumor Necrosis Factor Beta (TNFb)

LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha

  • 肿瘤坏死因子β(TNFb)活性蛋白 产品包装(模拟)
  • 肿瘤坏死因子β(TNFb)活性蛋白 产品包装(模拟)
  • APA134Hu01.jpg SDS-PAGE图
  • 肿瘤坏死因子β(TNFb)活性蛋白 Figure. Western Blot; Sample: APA134Hu01; Antibody: PAA134Hu01
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. The biological effects of TNF-β are very similar to TNF-α, due to the similarity of molecular structure and the receptors. As reported, TNF-β could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNF-β (10ng/mL) for 8h, 24h, 48h, 72h, then cells were observed by inverted microscope and IL-1β was detected in the cell supernatant by ELISA .
Cell necrosis after incubation with TNF-β(10ng/mL) for 72h was shown in Figure 1.

The production of IL-1β after incubation with TNF-β (10ng/mL) for 8h is shown in Table 1.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA134Hu01 肿瘤坏死因子β(TNFb)活性蛋白 Cell culture; Activity Assays.
RPA134Hu01 肿瘤坏死因子β(TNFb)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA134Hu01 肿瘤坏死因子β(TNFb)多克隆抗体 WB; IHC; ICC; IP.
MAA134Hu22 肿瘤坏死因子β(TNFb)单克隆抗体 WB; IHC; ICC; IP.
SEA134Hu 肿瘤坏死因子β(TNFb)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Journal of Applied Physiology Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process [PubMed: 22984252]
Journal of Translational Medicine Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantation [Pubmed:26847569]
International Journal of Stem Cell Research and Transplantation Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected Cytokines [IJST-2328-3548-04-803]
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