肿瘤坏死因子受体超家族成员12A(TNFRSF12A)活性蛋白
Active Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)
CD266; TNFRSF12-A; FN14; TWEAKR; Tumour Necrosis Factor Related Weak Inducer Of Apoptosis; Fibroblast growth factor-inducible immediate-early response protein 14
- 编号APA813Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 80%
- 等电点6.7
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
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产品包装(模拟)
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产品包装(模拟)
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SDS-PAGE图
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Figure. Western Blot; Sample: Recombinant TNFRSF12A, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA813Hu01 | 肿瘤坏死因子受体超家族成员12A(TNFRSF12A)活性蛋白 | Cell culture; Activity Assays. |
| RPA813Hu01 | 肿瘤坏死因子受体超家族成员12A(TNFRSF12A)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA813Hu01 | 肿瘤坏死因子受体超家族成员12A(TNFRSF12A)多克隆抗体 | WB; IHC; ICC; IP. |
| RAA813Hu21 | 肿瘤坏死因子受体超家族成员12A(TNFRSF12A)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAA813Hu21 | 肿瘤坏死因子受体超家族成员12A(TNFRSF12A)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA813Hu | 肿瘤坏死因子受体超家族成员12A(TNFRSF12A)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA813Hu | 肿瘤坏死因子受体超家族成员12A(TNFRSF12A)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Analytical Biochemistry | A single molecule assay for ultrasensitive detection of Fn14 in human serum [Pubmed: 31580829] |
