肿瘤坏死因子受体超家族成员1A(TNFRSF1A)活性蛋白

Active Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)

CD120A; P55; TBP1; FPF; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR1; TNFR55; TNFR60; P55-R; P60; Tumor necrosis factor receptor 1; Tumor necrosis factor-binding protein 1

  • 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)活性蛋白 产品包装(模拟)
  • 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)活性蛋白 产品包装(模拟)
  • APB499Hu01.png Figure. SDS-PAGE
  • 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The apoptosis of A549 cells by TNFα was inhibited by TNFRSF1B.
Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFα). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. TNFRSF1B can inhibit cell apoptosis which induced by TNFα. Briefly, A549 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1μg/mL TNFα and various concentrations of recombinant human TNFRSF1B. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate at 37°C for 1-4 hours. Apoptosis of A549 cells had been inhibit after incubation with TNFRSF1B for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant TNFRSF1B for 96h. The result was shown in Figure 2. It was obvious that TNFRSF1B significantly suppress cell apoptosis induced by TNFα.
(A) A549 cells cultured in DMEM contain 1μg/mL TNFα and 0.1ng/mL TNFRSF1B for 96h;
(B) A549 cells cultured in DMEM only contain 1μg/mL TNFα for 96h.

Figure. TNFRSF1B suppress the apoptosis of A549 cells induced by TNFα.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APB499Hu02 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)活性蛋白 Cell culture; Activity Assays.
RPB499Hu01 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB499Hu01 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)活性蛋白 Cell culture; Activity Assays.
RPB499Hu02 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB499Hu01 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)多克隆抗体 WB; IHC; ICC; IP.
PAB499Hu02 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)多克隆抗体 WB; IHC; ICC; IP.
MAB499Hu21 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)单克隆抗体 WB; IHC; ICC; IP.
SEB499Hu 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Mediators of Inflammation High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental Circulation [pubmed:28655972]
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