肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白

Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)

CD120B; P75; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR2; TNFR80; P75tnfr; Tumor necrosis factor receptor 2; p80 TNF-alpha receptor; Etanercept

  • 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白 产品包装(模拟)
  • 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白 产品包装(模拟)
  • 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白 Figure. Gene Sequencing (Extract)
  • APB504Hu01.png Figure. SDS-PAGE
  • 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of TNFRSF1B with TNFa.
Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFα). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Besides, Tumor Necrosis Factor Alpha (TNFa) has been identified as an interactor of TNFRSF1B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF1B and recombinant human TNFa. Briefly, TNFRSF1B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TNFRSF1B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF1B and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.

Figure. The apoptosis of A549 cells by TNFα was inhibited by TNFRSF1B.
TNFRSF1B can inhibit cell apoptosis which induced by TNFα. Briefly, A549 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well. and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1μg/mL TNFα and various concentrations of recombinant human TNFRSF1B. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate at 37°C for 1-4 hours. Apoptosis of A549 cells had been inhibit after incubation with TNFRSF1B for 96h observed by inverted microscope was shown in Figure 2. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant TNFRSF1B for 96h. The result was shown in Figure 2. It was obvious that TNFRSF1B significantly suppress cell apoptosis induced by TNFα.
(A) A549 cells cultured in DMEM contain 1μg/mL TNFα and 1ng/mL TNFRSF1B for 96h;
(B) A549 cells cultured in DMEM only contain 1μg/mL TNFα for 96h.

Figure.TNFRSF1B suppress apoptosis of A549 cells by TNFα.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

蛋白分子量MARKER
聚丙烯酰胺凝胶电泳(PAGE)实验服务
实时荧光定量PCR实验服务

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APB504Hu01 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白 Cell culture; Activity Assays.
RPB504Hu01 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB504Hu01 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)多克隆抗体 WB; IHC; ICC; IP.
MAB504Hu21 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)单克隆抗体 WB; IHC; ICC; IP.
SEB504Hu 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
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