肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白

Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)

CD120B; P75; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR2; TNFR80; P75tnfr; Tumor necrosis factor receptor 2; p80 TNF-alpha receptor; Etanercept

  • 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白 产品包装(模拟)
  • 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白 产品包装(模拟)
  • 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白 Figure. Gene Sequencing (Extract)
  • APB504Hu01.png Figure. SDS-PAGE
  • 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) , also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFα). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Besides, Tumor Necrosis Factor Alpha (TNFa) has been identified as an interactor of TNFRSF1B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF1B and recombinant human TNFa. Briefly, TNFRSF1B were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TNFa-coated microtiter wells and incubated for 1h at 37 ℃. Wells were washed with PBST and incubated for 1h with anti-TNFRSF1B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 ℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF1B and TNFa was shown in Figure 1, the EC50 was 0.0470~0.085 ug/ml.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

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编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
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