肿瘤坏死因子相关凋亡诱导配体(TRAIL)活性蛋白

Active Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)

CD253; TNFSF10; APO2L; Apo2-L; TL2; TRAIL; Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand

  • 肿瘤坏死因子相关凋亡诱导配体(TRAIL)活性蛋白 产品包装(模拟)
  • 肿瘤坏死因子相关凋亡诱导配体(TRAIL)活性蛋白 产品包装(模拟)
  • 肿瘤坏死因子相关凋亡诱导配体(TRAIL)活性蛋白 Gene sequencing
  • APA139Hu01.jpg SDS-PAGE图
  • 肿瘤坏死因子相关凋亡诱导配体(TRAIL)活性蛋白 Figure. Western Blot; Sample: Recombinant TRAIL, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Protein TRAIL (TNF-Related Apoptosis-Inducing Ligand) is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL induces apoptosis in transformed and tumor cells. This protein binds to several members of TNF receptor superfamily including TNFRSF10B/TRAILR2. Thus a binding ELISA assay was conducted to detect the interaction of TRAIL and TNFRSF10B. Briefly, recombinant human TRAIL were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFRSF10B-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TRAIL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TRAIL and TNFRSF10B was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

参考文献

杂志 参考文献
PLOS ONE Intranasal Administration of Recombinant TRAIL Down-Regulates CXCL-1/KC in an Ovalbumin-Induced Airway Inflammation Murine Model [Pubmed:25506835]
PLOS ONE Soluble TRAIL Concentration in Serum Is Elevated in People with Hypercholesterolemia [PubMed: 26633016]
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