肿瘤蛋白p53(P53)活性蛋白

Active Tumor Protein p53 (P53)

TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

  • 肿瘤蛋白p53(P53)活性蛋白 产品包装(模拟)
  • 肿瘤蛋白p53(P53)活性蛋白 产品包装(模拟)
  • APA928Ra01.png Figure. SDS-PAGE
  • 肿瘤蛋白p53(P53)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Tumor protein p53 (TP53), also known as p53, cellular tumor antigen p53 has many mechanisms of anticancer function and plays a role in apoptosis, genomic stability, and inhibition of angiogenesis. TP53 act as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It also Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. Besides, CREB Binding Protein (CREBBP) has been identified as an interactor of TP53, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TP53 and recombinant rat CREBBP. Briefly, TP53 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to CREBBP-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TP53 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TP53 and CREBBP was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of TP53 with CREBBP.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA928Ra01 肿瘤蛋白p53(P53)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA928Ra01 肿瘤蛋白p53(P53)活性蛋白 Cell culture; Activity Assays.
PAA928Ra01 肿瘤蛋白p53(P53)多克隆抗体 WB; IHC; ICC; IP.
MAA928Ra21 肿瘤蛋白p53(P53)单克隆抗体 WB; IHC; ICC; IP.
SEA928Ra 肿瘤蛋白p53(P53)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Anticancer Research Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines [PubMed: 23155233]
Microscopy Research and Technique Therapeutic role of curcumin in oxidative DNA damage caused by formaldehyde [PubMed: 25761397]
J Nutr Biochem Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing mice [PubMed: 26344777]
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